Detailed information of TA system TA210135

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 52635..53359
• Replicon: plasmid unnamed1
• Accession: NZ_CP079803
• Organism: Klebsiella variicola strain EMBL-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2J4YLV3
• Locus tag: KU883_RS27560
• Protein ID: WP_023292165.1
• Coordinates: 52635..52946 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KU883_RS27565
• Protein ID: WP_072122726.1
• Coordinates: 52943..53359 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU883_RS27540 (KU883_27525)	48642..49544	-	903	WP_060598839.1	LysR substrate-binding domain-containing protein	-
KU883_RS27545 (KU883_27530)	49827..50966	+	1140	WP_072122725.1	PLP-dependent aspartate aminotransferase family protein	-
KU883_RS27550 (KU883_27535)	50987..51916	+	930	WP_158236771.1	DMT family transporter	-
KU883_RS27555 (KU883_27540)	52032..52333	+	302	Protein_60	DDE-type integrase/transposase/recombinase	-
KU883_RS27560 (KU883_27545)	52635..52946	+	312	WP_023292165.1	type II toxin-antitoxin system HigB family toxin	Toxin
KU883_RS27565 (KU883_27550)	52943..53359	+	417	WP_072122726.1	helix-turn-helix domain-containing protein	Antitoxin
KU883_RS27570 (KU883_27555)	54176..54556	+	381	WP_077138875.1	hypothetical protein	-
KU883_RS27575 (KU883_27560)	54622..54969	+	348	WP_077138876.1	hypothetical protein	-
KU883_RS27580 (KU883_27565)	55057..55287	+	231	WP_004197732.1	hypothetical protein	-
KU883_RS27585 (KU883_27570)	55334..56167	+	834	WP_077138877.1	N-6 DNA methylase	-
KU883_RS27590 (KU883_27575)	56792..57322	+	531	WP_077138878.1	antirestriction protein	-
KU883_RS27595 (KU883_27580)	57360..57767	-	408	WP_224230374.1	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..98922	98922

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-133)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12497.32 Da
• Isoelectric Point: 9.9242

>T210135 WP_023292165.1 NZ_CP079803:52635-52946 [Klebsiella variicola]
VHVISRAPFDEAARHYPNDAAAIDDTYRVLKRVVAKKPDELKRYFTSLDRMKYREKWWVIDIGGNNLRMMFFADFERGKI
FVKHITTHAEYDRLTKYYREHKE

Nucleotide

• Length: 312 bp

>T210135 NZ_CP104405:c3865283-3864873 [Enterobacter hormaechei subsp. steigerwaltii]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGGCTGGTGGCGGC
ACTGGTGTTATTGGTGCCGTGGCCGCTTAGCTATACCCCTCTGTGGTTGCTATTGCTGTCGTTTGTGGTGTTTGACAGCG
TGCGCAGCCAGCGTCGAATCAACGCCCGTCAGGGGGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAGATGATGGGGACGCCGTGGATGCTTAATACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGATAACCGTCG
CCAGCACCTGTGGCTGGCCGCAGACAGTATGGATCCCGCGGAGTGGCGAGATCTGCGCCGGTTGATCGTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15468.55 Da
• Isoelectric Point: 4.4711

>AT210135 WP_072122726.1 NZ_CP079803:52943-53359 [Klebsiella variicola]
MIFSDAIKAANDLASIVPLLGGSSSRKDYEEALKLVEYLLEHDPDSPLVDMLTARIDTWEDNAVEFEEFNTRIEAGKNGV
SLLRVLMQQHGLSQSDFENEIGKKSLVSRILSGERSLTLDHMRALANRFQIPVSMFVD

Nucleotide

• Length: 417 bp

>AT210135 NZ_CP104405:c3865530-3865264 [Enterobacter hormaechei subsp. steigerwaltii]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A2J4YLV3