210110

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 170663..171248 Replicon plasmid pAJ_082-2
Accession NZ_CP079793
Organism Acinetobacter johnsonii strain AJ_082

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag EGT72_RS16390 Protein ID WP_219356867.1
Coordinates 170663..170983 (+) Length 107 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag EGT72_RS16395 Protein ID WP_000369781.1
Coordinates 170976..171248 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGT72_RS16350 (EGT72_016350) 166255..167058 + 804 WP_125281635.1 aminoglycoside O-phosphotransferase APH(3'')-Ib -
EGT72_RS16355 (EGT72_016355) 167058..167894 + 837 WP_125281636.1 APH(6)-I family aminoglycoside O-phosphotransferase -
EGT72_RS16360 (EGT72_016360) 168084..168371 + 288 WP_000438825.1 BrnT family toxin -
EGT72_RS16365 (EGT72_016365) 168358..168672 + 315 WP_004728120.1 BrnA antitoxin family protein -
EGT72_RS16370 (EGT72_016370) 168761..169291 - 531 WP_000172759.1 hypothetical protein -
EGT72_RS16375 (EGT72_016375) 169440..169742 + 303 WP_000286964.1 type II toxin-antitoxin system RelE/ParE family toxin -
EGT72_RS16380 (EGT72_016380) 169743..170024 + 282 WP_000985609.1 putative addiction module antidote protein -
EGT72_RS16385 (EGT72_016385) 170104..170523 - 420 WP_001180106.1 SMI1/KNR4 family protein -
EGT72_RS16390 (EGT72_016390) 170663..170983 + 321 WP_219356867.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EGT72_RS16395 (EGT72_016395) 170976..171248 + 273 WP_000369781.1 NadS family protein Antitoxin
EGT72_RS16400 (EGT72_016400) 171741..173216 + 1476 WP_000052512.1 ABC-F type ribosomal protection protein Msr(E) -
EGT72_RS16405 (EGT72_016405) 173272..174156 + 885 WP_000155092.1 Mph(E) family macrolide 2'-phosphotransferase -
EGT72_RS16410 (EGT72_016410) 174346..174957 - 612 WP_015060246.1 recombinase family protein -
EGT72_RS16415 (EGT72_016415) 175213..175629 + 417 WP_000147519.1 hypothetical protein -
EGT72_RS16420 (EGT72_016420) 175650..175892 + 243 WP_001229660.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul2 / aph(3'')-Ib / aph(6)-Id / msr(E) / mph(E) / blaNDM-1 / ARR-3 / qacE / sul1 / aph(3')-VIa - 1..340235 340235
- inside IScluster/Tn aph(3'')-Ib / aph(6)-Id / msr(E) / mph(E) - 159969..174156 14187


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(41-102)

Antitoxin

Cro/C1-type helix-turn-helix domain

(37-85)


Sequences

Toxin        


Download         Length: 107 a.a.        Molecular weight: 12159.94 Da        Isoelectric Point: 7.1637

>T210110 WP_219356867.1 NZ_CP079793:170663-170983 [Acinetobacter johnsonii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Download         Length: 321 bp

>T210110 NZ_CP104403:c368249-368070 [Enterobacter hormaechei subsp. steigerwaltii]
ATGGATAGCAGGAATGCAATAGCAATGATAGAAGCCGATGGGTGGTATCTGGTGAGAGTTAAAGGCAGTCATCATCAGTT
CAAACACCCAACGAAAAAGGGGCTGGTAACGGTAAAGCATCCACAGAAAGACATACCGTTACCAACACTGAAAAGCATCA
AAAAACAGGCGGGGCTCTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10149.61 Da        Isoelectric Point: 8.6582

>AT210110 WP_000369781.1 NZ_CP079793:170976-171248 [Acinetobacter johnsonii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Download         Length: 273 bp

>AT210110 NZ_CP104403:c368048-367638 [Enterobacter hormaechei subsp. steigerwaltii]
ATGTTGTACCCTGTTGCTATTGATAAAGGCGATTCATCCTTCGGCGTTCGCGTACCTGATATTCCTGGCTGCTTCTCTGG
CGGTGATGATTATCAGGACGCGATTGAAAGCGTACGTGAAGCGATCGAGGCACACATCGAATTACTGGTTGAAGATGGCG
AGAGCGTTCCAGAAGCAACGACCGTCGAAAACTGGCTTTCTGATCCAGAGTACGCCGGTGCGGTATGGGCCCTGGTTGAC
GTGGATATAACTCGTCTGATGGGAAAAGCGGAAAAAATCAATGTGACGCTTCCTTCACTGCTGATCCGACGTATCGATCA
GTTTGTGGCCGCCCATCCTGAATACGGTAGCCGGTCTGGCTTCCTGTCTCGTGTCGCGGCAGATAAAGTAATTGGACGAG
AAAAACGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References