Detailed information of TA system TA021010

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3376694..3377321
• Replicon: chromosome
• Accession: NC_005126
• Organism: Photorhabdus luminescens subsp. laumondii TTO1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q7N374
• Locus tag: PLU_RS14115
• Protein ID: WP_011147065.1
• Coordinates: 3376694..3377023 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PLU_RS14120
• Protein ID: WP_041380121.1
• Coordinates: 3377013..3377321 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PLU_RS14100 (plu2842)	3373241..3373873	+	633	WP_011147062.1	SDR family oxidoreductase	-
PLU_RS14105 (plu2843)	3373918..3374235	-	318	WP_011147063.1	DUF496 family protein	-
PLU_RS14110	3375437..3375667	+	231	WP_041380119.1	hypothetical protein	-
PLU_RS14115 (plu2845)	3376694..3377023	+	330	WP_011147065.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PLU_RS14120 (plu2846)	3377013..3377321	+	309	WP_041380121.1	helix-turn-helix domain-containing protein	Antitoxin
PLU_RS14125 (plu2847)	3377477..3378907	+	1431	WP_011147067.1	exodeoxyribonuclease I	-
PLU_RS14130 (plu2848)	3379035..3380750	-	1716	WP_011147068.1	D-lactate dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (15-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-97)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12899.94 Da
• Isoelectric Point: 9.7776

>T21010 WP_011147065.1 NC_005126:3376694-3377023 [Photorhabdus laumondii subsp. laumondii TTO1]
MKYTIYYYSTEVENEILALPDTLQARYIRYSERMREHGANLGEPHTEAFGNGLFELRLKGAEGIARVFYCTMIGHRIVML
HSFVKKTQKTPLKERRKAEVRMKEVKNDW

Nucleotide

• Length: 330 bp

>T21010 NC_005126:3376694-3377023 [Photorhabdus laumondii subsp. laumondii TTO1]
ATGAAATATACTATCTATTACTATAGCACCGAGGTGGAGAACGAGATCCTTGCTCTCCCTGACACACTTCAAGCCCGTTA
CATCAGGTATTCCGAAAGAATGCGAGAACATGGTGCCAACCTCGGAGAACCACACACAGAAGCGTTTGGTAATGGGTTGT
TTGAACTCAGACTCAAAGGGGCTGAAGGAATTGCTCGCGTATTCTATTGCACAATGATTGGGCACCGTATAGTGATGCTG
CACAGCTTCGTGAAGAAAACACAAAAGACCCCACTTAAAGAACGCAGGAAAGCCGAAGTCAGAATGAAAGAGGTAAAAAA
TGACTGGTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11170.03 Da
• Isoelectric Point: 10.2817

>AT21010 WP_041380121.1 NC_005126:3377013-3377321 [Photorhabdus laumondii subsp. laumondii TTO1]
MTGKYTPPTMTHDEMVAKMLSNPAVKAEYEKLEQEFALLDELLAARKKAGLTQAQVAERMGTKATAITRLESRLASGTQG
PSYNTLKKYAAAVGKKLQIRLV

Nucleotide

• Length: 309 bp

>AT21010 NC_005126:3377013-3377321 [Photorhabdus laumondii subsp. laumondii TTO1]
ATGACTGGTAAATATACCCCTCCAACCATGACACACGATGAAATGGTTGCAAAAATGTTGTCAAATCCGGCTGTAAAAGC
TGAATACGAGAAACTGGAACAAGAATTTGCATTGCTTGATGAACTGCTAGCTGCACGCAAAAAGGCCGGGCTAACACAAG
CGCAAGTAGCTGAACGCATGGGAACCAAAGCAACAGCCATCACACGGCTTGAAAGCCGGCTAGCTTCTGGCACACAAGGG
CCTTCGTATAATACATTGAAGAAATATGCAGCAGCTGTCGGTAAAAAATTACAGATACGCCTAGTATGA

Structures

Toxin

• AlphaFold DB: A0A6L9JI68