Detailed information of TA system TA021009

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2984444..2985048
• Replicon: chromosome
• Accession: NC_005126
• Organism: Photorhabdus luminescens subsp. laumondii TTO1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q7N418
• Locus tag: PLU_RS12580
• Protein ID: WP_011146760.1
• Coordinates: 2984737..2985048 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PLU_RS12575
• Protein ID: WP_011146759.1
• Coordinates: 2984444..2984734 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PLU_RS12555 (plu2531)	2980404..2980853	-	450	WP_011146754.1	phage tail protein	-
PLU_RS12560 (plu2532)	2981560..2982153	+	594	WP_011146755.1	winged helix-turn-helix domain-containing protein	-
PLU_RS12565 (plu2534)	2982682..2983026	+	345	WP_011146757.1	hypothetical protein	-
PLU_RS12570 (plu2535)	2983488..2984081	+	594	WP_011146758.1	winged helix-turn-helix domain-containing protein	-
PLU_RS12575 (plu2536)	2984444..2984734	-	291	WP_011146759.1	helix-turn-helix domain-containing protein	Antitoxin
PLU_RS12580 (plu2537)	2984737..2985048	-	312	WP_011146760.1	hypothetical protein	Toxin
PLU_RS12585 (plu2539)	2985502..2986272	-	771	WP_011146762.1	DUF2829 domain-containing protein	-
PLU_RS26050	2986641..2987479	-	839	Protein_2506	IS5 family transposase	-
PLU_RS25405 (plu2542)	2987529..2987786	-	258	WP_011146765.1	type II toxin-antitoxin system YafO family toxin	-
PLU_RS12600 (plu2543)	2987895..2988479	-	585	WP_011146766.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2961704..2994956	33252

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-87)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11976.74 Da
• Isoelectric Point: 8.0254

>T21009 WP_011146760.1 NC_005126:c2985048-2984737 [Photorhabdus laumondii subsp. laumondii TTO1]
MIFIETHIFTEDCKELLSDDEYREFQQYLADNPIAGDVIQHTGGLRKVRWASGSKGKRGGVRIIYYYKVDVSHIRLLLIY
KKGIKDDLSESEKKVLRALNEGW

Nucleotide

• Length: 312 bp

>T21009 NC_005126:c2985048-2984737 [Photorhabdus laumondii subsp. laumondii TTO1]
ATGATATTTATTGAGACCCATATCTTCACTGAAGATTGTAAAGAGTTGTTAAGTGACGATGAATATCGTGAATTTCAGCA
ATATCTCGCAGACAATCCTATTGCTGGTGATGTTATCCAACACACTGGTGGCTTACGTAAAGTCAGATGGGCATCTGGGA
GTAAAGGTAAGCGGGGTGGTGTGCGCATTATTTATTACTATAAAGTAGATGTCTCACACATCAGATTGTTACTGATTTAC
AAAAAAGGCATCAAGGATGACCTTAGTGAATCAGAAAAGAAAGTCTTGAGAGCACTAAATGAGGGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10645.32 Da
• Isoelectric Point: 10.7721

>AT21009 WP_011146759.1 NC_005126:c2984734-2984444 [Photorhabdus laumondii subsp. laumondii TTO1]
MDSKLFSRLTESMTQMNEIINGERAASRETHVEAVKVKNIRQATGLTQTGFAKLISVNVGTLRNWEQGRREPTGPAKALL
KAIEKDPVHVLKALSM

Nucleotide

• Length: 291 bp

>AT21009 NC_005126:c2984734-2984444 [Photorhabdus laumondii subsp. laumondii TTO1]
ATGGATAGTAAATTATTTTCTAGACTAACTGAAAGCATGACACAGATGAACGAAATTATTAATGGCGAACGTGCTGCCTC
TCGTGAAACTCACGTTGAAGCAGTAAAAGTAAAGAATATTCGCCAGGCAACCGGTTTAACTCAGACCGGTTTTGCTAAGT
TGATTTCCGTTAATGTTGGAACTCTGCGCAACTGGGAACAGGGAAGGCGTGAGCCAACCGGACCAGCAAAAGCCCTGTTG
AAGGCAATAGAGAAAGATCCGGTTCATGTTTTAAAGGCGTTATCTATGTAA

Structures

Toxin

• AlphaFold DB: A0A6L9JR19