Detailed information of TA system TA210080

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3924255..3924949
• Replicon: chromosome
• Accession: NZ_CP079780
• Organism: Escherichia coli strain BL12

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: KYX59_RS21140
• Protein ID: WP_259481870.1
• Coordinates: 3924551..3924949 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: KYX59_RS21135
• Protein ID: WP_000554757.1
• Coordinates: 3924255..3924548 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYX59_RS21115 (3919887)	3919887..3920384	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
KYX59_RS21120 (3920608)	3920608..3922320	-	1713	Protein_3733	flagellar biosynthesis protein FlhA	-
KYX59_RS21125 (3922292)	3922292..3923077	+	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
KYX59_RS21130 (3923148)	3923148..3924203	+	1056	WP_001226164.1	DNA polymerase IV	-
KYX59_RS21135 (3924255)	3924255..3924548	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KYX59_RS21140 (3924551)	3924551..3924949	+	399	WP_259481870.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KYX59_RS21145 (3924959)	3924959..3925411	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
KYX59_RS21150 (3925657)	3925657..3925860	+	204	Protein_3739	RtcB family protein	-
KYX59_RS21155 (3925859)	3925859..3926380	+	522	Protein_3740	peptide chain release factor H	-
KYX59_RS21160 (3926437)	3926437..3927894	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
KYX59_RS21165 (3928155)	3928155..3928613	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (3929209)	3929209..3929289	+	81	NuclAT_10	-	-
- (3929209)	3929209..3929289	+	81	NuclAT_10	-	-
- (3929209)	3929209..3929289	+	81	NuclAT_10	-	-
- (3929209)	3929209..3929289	+	81	NuclAT_10	-	-
KYX59_RS21170 (3928705)	3928705..3929949	+	1245	WP_000189541.1	esterase FrsA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15458.80 Da
• Isoelectric Point: 8.0949

>T210080 WP_259481870.1 NZ_CP079780:3924551-3924949 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDDQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T210080 NZ_CP079780:3924551-3924949 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGATCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT210080 WP_000554757.1 NZ_CP079780:3924255-3924548 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT210080 NZ_CP079780:3924255-3924548 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6