Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3918211..3918752 Replicon chromosome
Accession NZ_CP079780
Organism Escherichia coli strain BL12

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag KYX59_RS21100 Protein ID WP_000615976.1
Coordinates 3918211..3918489 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag KYX59_RS21105 Protein ID WP_000729704.1
Coordinates 3918492..3918752 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYX59_RS21085 (3915793) 3915793..3916371 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
KYX59_RS21090 (3916577) 3916577..3917344 + 768 WP_000333379.1 class II glutamine amidotransferase -
KYX59_RS21095 (3917315) 3917315..3918055 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KYX59_RS21100 (3918211) 3918211..3918489 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KYX59_RS21105 (3918492) 3918492..3918752 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KYX59_RS21110 (3918938) 3918938..3919711 + 774 WP_015675129.1 C40 family peptidase -
KYX59_RS21115 (3919887) 3919887..3920384 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
KYX59_RS21120 (3920608) 3920608..3922320 - 1713 Protein_3733 flagellar biosynthesis protein FlhA -
KYX59_RS21125 (3922292) 3922292..3923077 + 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA 3893033..3924203 31170


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T210079 WP_000615976.1 NZ_CP079780:c3918489-3918211 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T210079 NZ_CP104384:c4283066-4282893 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT210079 WP_000729704.1 NZ_CP079780:c3918752-3918492 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT210079 NZ_CP104384:c4283390-4283094 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References