Detailed information of TA system TA210075

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2299011..2299810
• Replicon: chromosome
• Accession: NZ_CP079780
• Organism: Escherichia coli strain BL12

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: KYX59_RS13420
• Protein ID: WP_032221941.1
• Coordinates: 2299346..2299810 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: KYX59_RS13415
• Protein ID: WP_001307405.1
• Coordinates: 2299011..2299346 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYX59_RS13400 (2294796)	2294796..2295566	-	771	WP_001723934.1	2-dehydro-3-deoxyglucarate aldolase	-
KYX59_RS13405 (2295582)	2295582..2296916	-	1335	WP_001723935.1	galactarate/glucarate/glycerate transporter GarP	-
KYX59_RS13410 (2297291)	2297291..2298862	+	1572	WP_001723936.1	galactarate dehydratase	-
KYX59_RS13415 (2299011)	2299011..2299346	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
KYX59_RS13420 (2299346)	2299346..2299810	+	465	WP_032221941.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
KYX59_RS13425 (2299865)	2299865..2300674	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
KYX59_RS13430 (2300923)	2300923..2302203	+	1281	WP_000681903.1	tagatose-bisphosphate aldolase subunit KbaZ	-
KYX59_RS13435 (2302226)	2302226..2302699	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
KYX59_RS13440 (2302710)	2302710..2303489	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
KYX59_RS13445 (2303479)	2303479..2304357	+	879	WP_001300474.1	PTS N-acetylgalactosamine transporter subunit IID	-
KYX59_RS13450 (2304375)	2304375..2304809	+	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17808.24 Da
• Isoelectric Point: 9.6804

>T210075 WP_032221941.1 NZ_CP079780:2299346-2299810 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQKKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T210075 NZ_CP104384:c3676257-3675880 [Escherichia coli]
CTGAAAACATTACCTGACACACACGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTGCGCACCGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATCAACAGTATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCGGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT210075 WP_001307405.1 NZ_CP079780:2299011-2299346 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT210075 NZ_CP104384:c3676678-3676304 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGAGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0XVC7