Detailed information of TA system TA210040

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 24005..24431
• Replicon: plasmid pGS04EC-2
• Accession: NZ_CP079774
• Organism: Escherichia coli strain GS04

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KYX57_RS23250
• Protein ID: WP_001372321.1
• Coordinates: 24005..24130 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	24207..24431 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYX57_RS23220 (19383)	19383..20072	-	690	WP_000283380.1	conjugal transfer transcriptional regulator TraJ	-
KYX57_RS23225 (20259)	20259..20642	-	384	WP_053320747.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KYX57_RS23230 (20963)	20963..21565	+	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
KYX57_RS23235 (21862)	21862..22683	-	822	WP_001234445.1	DUF932 domain-containing protein	-
KYX57_RS23240 (22794)	22794..23090	-	297	WP_001272251.1	hypothetical protein	-
KYX57_RS23245 (23390)	23390..23686	+	297	Protein_28	hypothetical protein	-
KYX57_RS23250 (24005)	24005..24130	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KYX57_RS23255 (24072)	24072..24221	-	150	Protein_30	plasmid maintenance protein Mok	-
KYX57_RS23260 (24243)	24243..24422	+	180	WP_001309233.1	hypothetical protein	-
- (24207)	24207..24431	-	225	NuclAT_0	-	Antitoxin
- (24207)	24207..24431	-	225	NuclAT_0	-	Antitoxin
- (24207)	24207..24431	-	225	NuclAT_0	-	Antitoxin
- (24207)	24207..24431	-	225	NuclAT_0	-	Antitoxin
KYX57_RS23265 (24400)	24400..25162	-	763	Protein_32	plasmid SOS inhibition protein A	-
KYX57_RS23270 (25159)	25159..25593	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
KYX57_RS23275 (25662)	25662..27685	-	2024	Protein_34	ParB/RepB/Spo0J family partition protein	-
KYX57_RS23280 (27746)	27746..27979	-	234	WP_000006003.1	DUF905 family protein	-
KYX57_RS23285 (28037)	28037..28564	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
KYX57_RS23290 (28866)	28866..29321	+	456	Protein_37	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T210040 WP_001372321.1 NZ_CP079774:c24130-24005 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T210040 NZ_CP104371:3530816-3531166 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT210040 NZ_CP079774:c24431-24207 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin