Detailed information of TA system TA210033

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4150613..4151412
• Replicon: chromosome
• Accession: NZ_CP079772
• Organism: Escherichia coli strain GS04

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: KYX57_RS20300
• Protein ID: WP_032221941.1
• Coordinates: 4150613..4151077 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: KYX57_RS20305
• Protein ID: WP_001307405.1
• Coordinates: 4151077..4151412 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYX57_RS20270 (4145614)	4145614..4146048	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
KYX57_RS20275 (4146066)	4146066..4146944	-	879	WP_001300474.1	PTS N-acetylgalactosamine transporter subunit IID	-
KYX57_RS20280 (4146934)	4146934..4147713	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
KYX57_RS20285 (4147724)	4147724..4148197	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
KYX57_RS20290 (4148220)	4148220..4149500	-	1281	WP_000681903.1	tagatose-bisphosphate aldolase subunit KbaZ	-
KYX57_RS20295 (4149749)	4149749..4150558	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
KYX57_RS20300 (4150613)	4150613..4151077	-	465	WP_032221941.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
KYX57_RS20305 (4151077)	4151077..4151412	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
KYX57_RS20310 (4151561)	4151561..4153132	-	1572	WP_001723936.1	galactarate dehydratase	-
KYX57_RS20315 (4153507)	4153507..4154841	+	1335	WP_001723935.1	galactarate/glucarate/glycerate transporter GarP	-
KYX57_RS20320 (4154857)	4154857..4155627	+	771	WP_001723934.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17808.24 Da
• Isoelectric Point: 9.6804

>T210033 WP_032221941.1 NZ_CP079772:c4151077-4150613 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQKKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T210033 NZ_CP104371:c1027693-1027310 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGTTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGTGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCG
GGACAATTACAGAATGGTAAATAACATTACCCTGGGCAAGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT210033 WP_001307405.1 NZ_CP079772:c4151412-4151077 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT210033 NZ_CP104371:c1028154-1027774 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGGCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0XVC7