Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2531670..2532211 Replicon chromosome
Accession NZ_CP079772
Organism Escherichia coli strain GS04

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag KYX57_RS12630 Protein ID WP_000615976.1
Coordinates 2531933..2532211 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag KYX57_RS12625 Protein ID WP_000729704.1
Coordinates 2531670..2531930 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYX57_RS12605 (2527345) 2527345..2528130 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
KYX57_RS12610 (2528102) 2528102..2529814 + 1713 Protein_2470 flagellar biosynthesis protein FlhA -
KYX57_RS12615 (2530038) 2530038..2530535 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
KYX57_RS12620 (2530711) 2530711..2531484 - 774 WP_015675129.1 C40 family peptidase -
KYX57_RS12625 (2531670) 2531670..2531930 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KYX57_RS12630 (2531933) 2531933..2532211 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KYX57_RS12635 (2532367) 2532367..2533107 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KYX57_RS12640 (2533078) 2533078..2533845 - 768 WP_000333379.1 class II glutamine amidotransferase -
KYX57_RS12645 (2534051) 2534051..2534629 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T210029 WP_000615976.1 NZ_CP079772:2531933-2532211 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T210029 NZ_CP104371:c684720-684544 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCAATGTAGCAAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTACGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT210029 WP_000729704.1 NZ_CP079772:2531670-2531930 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT210029 NZ_CP104371:c684498-684082 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGACATCACTCAGCAAGAATTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References