Detailed information of TA system TA210028

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2525473..2526167
• Replicon: chromosome
• Accession: NZ_CP079772
• Organism: Escherichia coli strain GS04

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: KYX57_RS12590
• Protein ID: WP_259481870.1
• Coordinates: 2525473..2525871 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: KYX57_RS12595
• Protein ID: WP_000554757.1
• Coordinates: 2525874..2526167 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (2521133)	2521133..2521213	-	81	NuclAT_10	-	-
- (2521133)	2521133..2521213	-	81	NuclAT_10	-	-
- (2521133)	2521133..2521213	-	81	NuclAT_10	-	-
- (2521133)	2521133..2521213	-	81	NuclAT_10	-	-
KYX57_RS12560 (2520473)	2520473..2521717	-	1245	WP_000189541.1	esterase FrsA	-
KYX57_RS12565 (2521809)	2521809..2522267	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
KYX57_RS12570 (2522528)	2522528..2523985	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
KYX57_RS12575 (2524042)	2524042..2524563	-	522	Protein_2463	peptide chain release factor H	-
KYX57_RS12580 (2524562)	2524562..2524765	-	204	Protein_2464	RtcB family protein	-
KYX57_RS12585 (2525011)	2525011..2525463	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
KYX57_RS12590 (2525473)	2525473..2525871	-	399	WP_259481870.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KYX57_RS12595 (2525874)	2525874..2526167	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KYX57_RS12600 (2526219)	2526219..2527274	-	1056	WP_001226164.1	DNA polymerase IV	-
KYX57_RS12605 (2527345)	2527345..2528130	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
KYX57_RS12610 (2528102)	2528102..2529814	+	1713	Protein_2470	flagellar biosynthesis protein FlhA	-
KYX57_RS12615 (2530038)	2530038..2530535	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15458.80 Da
• Isoelectric Point: 8.0949

>T210028 WP_259481870.1 NZ_CP079772:c2525871-2525473 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDDQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T210028 NZ_CP104371:636674-636952 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCATCGACTTGCTGTGATTGATGCGGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT210028 WP_000554757.1 NZ_CP079772:c2526167-2525874 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT210028 NZ_CP104371:636952-637236 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGTCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGATTACGCCGTCTGGTTACGCAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6