Detailed information of TA system TA210011

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 4196459..4197152
• Replicon: chromosome
• Accession: NZ_CP079770
• Organism: Escherichia coli strain HL15

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: KYX58_RS22200
• Protein ID: WP_000415584.1
• Coordinates: 4196459..4196755 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: KYX58_RS22205
• Protein ID: WP_000650107.1
• Coordinates: 4196757..4197152 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYX58_RS22165 (4191547)	4191547..4191861	-	315	WP_000958598.1	putative quinol monooxygenase	-
KYX58_RS22170 (4191892)	4191892..4192473	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
KYX58_RS22175 (4192792)	4192792..4193124	+	333	WP_000917684.1	DUF2645 family protein	-
KYX58_RS22180 (4193170)	4193170..4194519	-	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
KYX58_RS22185 (4194516)	4194516..4195175	-	660	WP_001221495.1	quorum sensing response regulator transcription factor QseB	-
KYX58_RS22190 (4195327)	4195327..4195719	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
KYX58_RS22195 (4195772)	4195772..4196254	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
KYX58_RS22200 (4196459)	4196459..4196755	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
KYX58_RS22205 (4196757)	4196757..4197152	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
KYX58_RS22210 (4197285)	4197285..4198892	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
KYX58_RS22215 (4199030)	4199030..4201288	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	rfaE	4136906..4201288	64382

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T210011 WP_000415584.1 NZ_CP079770:4196459-4196755 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T210011 NZ_CP104366:838230-838643 [Salmonella enterica subsp. enterica serovar Typhimurium]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT210011 WP_000650107.1 NZ_CP079770:4196757-4197152 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT210011 NZ_CP104366:837983-838249 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65