Detailed information of TA system TA021000

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 2485287..2485937
• Replicon: chromosome
• Accession: NC_005126
• Organism: Photorhabdus luminescens subsp. laumondii TTO1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q7N558
• Locus tag: PLU_RS10490
• Protein ID: WP_011146354.1
• Coordinates: 2485602..2485937 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PLU_RS10485
• Protein ID: WP_011146353.1
• Coordinates: 2485287..2485601 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PLU_RS10460 (plu2093)	2480557..2481294	-	738	WP_011146348.1	cyclase family protein	-
PLU_RS10465 (plu2094)	2481898..2482452	+	555	WP_011146349.1	cytochrome b	-
PLU_RS10470 (plu2095)	2482479..2483057	+	579	WP_011146350.1	YceI family protein	-
PLU_RS10475 (plu2096)	2483371..2483739	-	369	WP_011146351.1	hypothetical protein	-
PLU_RS10480 (plu2097)	2484708..2485271	+	564	WP_011146352.1	VOC family protein	-
PLU_RS10485 (plu2098)	2485287..2485601	-	315	WP_011146353.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
PLU_RS10490 (plu2099)	2485602..2485937	-	336	WP_011146354.1	hypothetical protein	Toxin
PLU_RS25280 (plu2100)	2485992..2486150	+	159	Protein_2070	VOC family protein	-
PLU_RS10495 (plu2101)	2486288..2487034	+	747	WP_011146355.1	copper homeostasis protein CutC	-
PLU_RS10500 (plu2102)	2487210..2488181	-	972	WP_011146356.1	tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB	-
PLU_RS10505 (plu2103)	2488178..2488936	-	759	WP_011146357.1	carboxy-S-adenosyl-L-methionine synthase CmoA	-
PLU_RS10510 (plu2104)	2489209..2489460	+	252	WP_011146358.1	plasmid stabilization protein	-
PLU_RS10515 (plu2105)	2489450..2489740	+	291	WP_011146359.1	type II toxin-antitoxin system RelE/ParE family toxin	-
PLU_RS10520 (plu2106)	2489867..2490262	-	396	WP_011146360.1	MAPEG family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Antitoxin MqsA/HigA-2 (13-98)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 13393.51 Da
• Isoelectric Point: 10.1960

>T21000 WP_011146354.1 NC_005126:c2485937-2485602 [Photorhabdus laumondii subsp. laumondii TTO1]
MEAVFIELPPFERYRHNYLSDEEFRTFQNMLLKNPTTGDVIQHTGGLRKVRFTDSRRKKGKRGGIRIIYYWWVSKSQFML
FTIYDKDEMSDLTKEQCESLRKMLEMRKKGA

Nucleotide

• Length: 336 bp

>T21000 NC_005126:c2485937-2485602 [Photorhabdus laumondii subsp. laumondii TTO1]
ATGGAAGCTGTTTTTATTGAACTACCACCTTTTGAACGTTACCGTCATAACTACCTGAGTGATGAGGAGTTTCGAACCTT
CCAAAATATGCTACTGAAAAATCCAACAACAGGAGATGTAATCCAACATACAGGCGGATTGCGTAAAGTACGTTTTACTG
ATTCCCGAAGGAAAAAGGGAAAACGAGGCGGTATTCGGATAATTTACTATTGGTGGGTATCTAAATCGCAATTCATGCTT
TTCACTATCTATGATAAAGATGAAATGTCAGACTTAACTAAAGAGCAATGCGAATCCTTGCGTAAAATGTTAGAAATGAG
AAAAAAAGGAGCATAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12059.89 Da
• Isoelectric Point: 10.1310

>AT21000 WP_011146353.1 NC_005126:c2485601-2485287 [Photorhabdus laumondii subsp. laumondii TTO1]
MKRDLFAELNEGMKAWEEANEGKLTLKTHKRRLKNDIFISPDELKSIRSKLNISQAVFAQYLHTGETTYQNWEQGRAKPN
KQAVLLIKMVERNPQALEFLASLT

Nucleotide

• Length: 315 bp

>AT21000 NC_005126:c2485601-2485287 [Photorhabdus laumondii subsp. laumondii TTO1]
ATGAAGCGTGATCTATTTGCAGAACTCAATGAAGGGATGAAAGCTTGGGAAGAGGCTAACGAAGGCAAACTCACATTGAA
AACTCATAAGAGGAGGTTAAAAAATGATATCTTCATCAGTCCGGATGAGTTGAAATCTATCCGGAGTAAACTGAATATTT
CTCAGGCTGTTTTTGCCCAGTACCTGCATACCGGTGAGACGACATACCAAAACTGGGAACAGGGACGGGCTAAACCGAAT
AAACAAGCTGTGTTACTGATTAAAATGGTGGAGCGCAACCCGCAGGCACTAGAATTTCTTGCCAGCCTCACATAA

Structures

Toxin

• AlphaFold DB: A0A6L9JGM0