Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4087969..4088696 Replicon chromosome
Accession NZ_CP079767
Organism Escherichia coli strain SF10

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag KX927_RS22345 Protein ID WP_000550189.1
Coordinates 4087969..4088283 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KX927_RS22350 Protein ID WP_000560266.1
Coordinates 4088280..4088696 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KX927_RS22325 (4084127) 4084127..4085113 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
KX927_RS22330 (4085192) 4085192..4085884 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
KX927_RS22335 (4085961) 4085961..4086464 - 504 WP_001333820.1 M48 family metallopeptidase -
KX927_RS22340 (4086549) 4086549..4087685 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
KX927_RS22345 (4087969) 4087969..4088283 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
KX927_RS22350 (4088280) 4088280..4088696 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
KX927_RS22355 (4088741) 4088741..4090759 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
KX927_RS22360 (4091185) 4091185..4093536 - 2352 WP_259553597.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T209984 WP_000550189.1 NZ_CP079767:4087969-4088283 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T209984 NZ_CP104358:c3987797-3987510 [Salmonella enterica]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGCTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCTCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT209984 WP_000560266.1 NZ_CP079767:4088280-4088696 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT209984 NZ_CP104358:c3987523-3987221 [Salmonella enterica]
ATGAGCATGGTTAAACATAAACGCGGCAATGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References