Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4961429..4962026 Replicon chromosome
Accession NZ_CP079762
Organism Klebsiella pneumoniae strain JP20

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KYE76_RS24035 Protein ID WP_004142563.1
Coordinates 4961429..4961746 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KYE76_RS24040 Protein ID WP_004142561.1
Coordinates 4961739..4962026 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYE76_RS24020 (4957129) 4957129..4958556 - 1428 WP_009308097.1 MFS transporter -
KYE76_RS24025 (4958665) 4958665..4959531 + 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
KYE76_RS24030 (4960201) 4960201..4961244 + 1044 WP_020325241.1 DUF2157 domain-containing protein -
KYE76_RS24035 (4961429) 4961429..4961746 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KYE76_RS24040 (4961739) 4961739..4962026 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KYE76_RS24045 (4962291) 4962291..4962944 - 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
KYE76_RS24050 (4963064) 4963064..4963432 - 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
KYE76_RS24055 (4963432) 4963432..4964013 - 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
KYE76_RS24060 (4964193) 4964193..4965308 + 1116 WP_020325248.1 MBL fold metallo-hydrolase -
KYE76_RS24065 (4965339) 4965339..4965680 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KYE76_RS24070 (4965698) 4965698..4965946 - 249 WP_020325243.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T209962 WP_004142563.1 NZ_CP079762:4961429-4961746 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T209962 NZ_CP104355:661004-661291 [Vibrio cholerae]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT209962 WP_004142561.1 NZ_CP079762:4961739-4962026 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT209962 NZ_CP104355:661302-661619 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTGAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACCCCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References