Detailed information of TA system TA020996

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 993019..993589
• Replicon: chromosome
• Accession: NC_005126
• Organism: Photorhabdus luminescens subsp. laumondii TTO1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q7N875
• Locus tag: PLU_RS04315
• Protein ID: WP_011145234.1
• Coordinates: 993308..993589 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PLU_RS04310
• Protein ID: WP_011145233.1
• Coordinates: 993019..993297 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PLU_RS04280 (plu0859)	989172..989435	+	264	WP_011145227.1	helix-turn-helix domain-containing protein	-
PLU_RS04285 (plu0860)	989805..990350	+	546	WP_011145228.1	hypoxanthine phosphoribosyltransferase	-
PLU_RS04290 (plu0861)	990571..990849	+	279	WP_011145229.1	DUF1778 domain-containing protein	-
PLU_RS04295 (plu0862)	990854..991354	+	501	WP_011145230.1	GNAT family N-acetyltransferase	-
PLU_RS04300 (plu0863)	991509..992048	+	540	WP_041379935.1	SocA family protein	-
PLU_RS04305 (plu0864)	992383..992919	+	537	WP_011145232.1	hypoxanthine phosphoribosyltransferase	-
PLU_RS04310 (plu0865)	993019..993297	-	279	WP_011145233.1	HigA family addiction module antidote protein	Antitoxin
PLU_RS04315 (plu0866)	993308..993589	-	282	WP_011145234.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PLU_RS04320 (plu0867)	995109..995762	-	654	WP_011145235.1	carbonate dehydratase	-
PLU_RS04325 (plu0868)	995950..996861	+	912	WP_011145236.1	ABC transporter ATP-binding protein	-
PLU_RS04330 (plu0869)	996873..997643	+	771	WP_011145237.1	ABC transporter permease	-
PLU_RS04335 (plu0870)	997722..998102	-	381	WP_011145238.1	aspartate 1-decarboxylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-63)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10633.28 Da
• Isoelectric Point: 10.4815

>T20996 WP_011145234.1 NC_005126:c993589-993308 [Photorhabdus laumondii subsp. laumondii TTO1]
MIKSFKHKGLEKFFKTGSTAGIQPKHALKLQIQLTALNTAKKPEDMNAPSWKLHPLKGANLSGHWAISVNSNWRLTFRFE
GEDIILVNYQDYH

Nucleotide

• Length: 282 bp

>T20996 NC_005126:c993589-993308 [Photorhabdus laumondii subsp. laumondii TTO1]
ATGATTAAAAGCTTCAAACACAAAGGATTGGAAAAATTTTTCAAAACTGGTTCTACCGCTGGCATTCAGCCTAAGCATGC
CCTAAAGCTGCAAATCCAGCTAACTGCATTGAATACGGCTAAAAAACCAGAAGATATGAATGCCCCTAGCTGGAAACTTC
ACCCACTAAAAGGAGCAAATCTTAGCGGTCACTGGGCAATATCTGTTAACAGTAATTGGCGTTTAACATTTAGATTTGAG
GGCGAAGATATAATTCTCGTTAATTATCAAGATTATCACTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 9992.63 Da
• Isoelectric Point: 9.7351

>AT20996 WP_011145233.1 NC_005126:c993297-993019 [Photorhabdus laumondii subsp. laumondii TTO1]
MKMHKPAHPGIVLREYLGDISVTEAAKALGITRAALSRILNGNAGISADMALRLEAALGTSAEMWTGMQSQYELWIASQH
KRPEIKPILAHS

Nucleotide

• Length: 279 bp

>AT20996 NC_005126:c993297-993019 [Photorhabdus laumondii subsp. laumondii TTO1]
ATGAAAATGCATAAACCTGCACACCCAGGCATAGTTTTGCGTGAATATCTTGGTGACATATCTGTCACGGAAGCAGCAAA
GGCTCTCGGTATAACCAGAGCTGCACTATCCCGCATATTAAACGGCAATGCAGGAATATCTGCTGATATGGCCCTGCGAC
TGGAAGCCGCATTAGGTACTAGTGCCGAAATGTGGACAGGAATGCAGTCCCAATATGAACTGTGGATTGCCTCACAGCAC
AAAAGACCAGAAATAAAACCAATACTGGCTCACTCCTGA

Structures

Toxin

• AlphaFold DB: Q7N875