Detailed information of TA system TA209932

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5224728..5225323
• Replicon: chromosome
• Accession: NZ_CP079758
• Organism: Pseudomonas aeruginosa strain PA0386

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: KYE95_RS24255
• Protein ID: WP_003113526.1
• Coordinates: 5225045..5225323 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KYE95_RS24250
• Protein ID: WP_003113527.1
• Coordinates: 5224728..5225033 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYE95_RS24215 (KYE95_24130)	5219867..5220715	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
KYE95_RS24225 (KYE95_24140)	5220882..5221823	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
KYE95_RS24230 (KYE95_24145)	5221940..5222554	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
KYE95_RS24235 (KYE95_24150)	5222596..5223180	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
KYE95_RS24240 (KYE95_24155)	5223221..5224321	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
KYE95_RS24250 (KYE95_24165)	5224728..5225033	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
KYE95_RS24255 (KYE95_24170)	5225045..5225323	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KYE95_RS24260	5225376..5225504	-	129	Protein_4790	integrase	-
KYE95_RS24265 (KYE95_24175)	5225652..5227880	+	2229	WP_003113525.1	TonB-dependent receptor	-
KYE95_RS24270 (KYE95_24180)	5227950..5228597	-	648	WP_003095021.1	carbonate dehydratase	-
KYE95_RS24275 (KYE95_24185)	5228659..5229897	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T209932 WP_003113526.1 NZ_CP079758:c5225323-5225045 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T209932 NZ_CP104343:c204192-203905 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT209932 WP_003113527.1 NZ_CP079758:c5225033-5224728 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT209932 NZ_CP104343:c203918-203604 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: V6ALY3