Detailed information of TA system TA209917

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 91278..92005
• Replicon: plasmid pNY1A
• Accession: NZ_CP079753
• Organism: Raoultella ornithinolytica strain NY1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5Q9LNL2
• Locus tag: KXJ76_RS26800
• Protein ID: WP_004118600.1
• Coordinates: 91278..91589 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: V0AHC4
• Locus tag: KXJ76_RS26805
• Protein ID: WP_000990392.1
• Coordinates: 91586..92005 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXJ76_RS26780 (KXJ76_26780)	87792..88511	+	720	WP_001102107.1	(Fe-S)-binding protein	-
KXJ76_RS26785 (KXJ76_26785)	88522..89949	+	1428	WP_000023895.1	LutB/LldF family L-lactate oxidation iron-sulfur protein	-
KXJ76_RS26790 (KXJ76_26790)	89942..90637	+	696	WP_004118595.1	lactate utilization protein C	-
KXJ76_RS26795 (KXJ76_26795)	90687..91067	-	381	Protein_97	gluconate permease	-
KXJ76_RS26800 (KXJ76_26800)	91278..91589	+	312	WP_004118600.1	type II toxin-antitoxin system HigB family toxin	Toxin
KXJ76_RS26805 (KXJ76_26805)	91586..92005	+	420	WP_000990392.1	helix-turn-helix domain-containing protein	Antitoxin
KXJ76_RS26810 (KXJ76_26810)	92042..93244	-	1203	WP_003031541.1	hypothetical protein	-
KXJ76_RS26815 (KXJ76_26815)	93237..93566	-	330	WP_004118613.1	hypothetical protein	-
KXJ76_RS26820 (KXJ76_26820)	93563..94222	-	660	WP_000078540.1	hypothetical protein	-
KXJ76_RS27375	94646..95002	-	357	WP_077253206.1	Ref family recombination enhancement nuclease	-
KXJ76_RS27380	95122..95262	-	141	WP_004118614.1	hypothetical protein	-
KXJ76_RS26830 (KXJ76_26830)	95555..95908	+	354	WP_001114073.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
KXJ76_RS26835 (KXJ76_26835)	95956..96318	+	363	WP_000783215.1	arsenite efflux transporter metallochaperone ArsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..167012	167012

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12386.14 Da
• Isoelectric Point: 10.0935

>T209917 WP_004118600.1 NZ_CP079753:91278-91589 [Raoultella ornithinolytica]
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Nucleotide

• Length: 312 bp

>T209917 NZ_CP104336:c291431-291129 [Acinetobacter baumannii]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTCACCAAGTTAAAAGACCAGCAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAGCCGGTCGGTGAAGGTGTTTCTGAATTACGTTTCT
TCTTTGGGCCAGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTTGTTATTCTTTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTGCAATTGGCACAAGATTTAGAGGAGGAGCTATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15534.71 Da
• Isoelectric Point: 4.4702

>AT209917 WP_000990392.1 NZ_CP079753:91586-92005 [Raoultella ornithinolytica]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Nucleotide

• Length: 420 bp

>AT209917 NZ_CP104336:c291128-290847 [Acinetobacter baumannii]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAGCATCTTAAAACAGAGGATGATATGCAGGCTTACTTACAAGCATG
TATTGAAGAATCTAATGGCGATGCCGCATTCATTGCAAAGGCTTTAGGTAACATTGCAAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGCTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Structures

Toxin

• AlphaFold DB: A0A5Q9LNL2

Antitoxin

• AlphaFold DB: V0AHC4