Detailed information of TA system TA209893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 30646..31231
• Replicon: plasmid pMRA
• Accession: NZ_CP079749
• Organism: Acinetobacter johnsonii strain MR1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KXJ74_RS17580
• Protein ID: WP_219191782.1
• Coordinates: 30646..30966 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KXJ74_RS17585
• Protein ID: WP_219191783.1
• Coordinates: 30959..31231 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXJ74_RS17565 (KXJ74_17565)	27778..28149	+	372	WP_024160689.1	transposase family protein	-
KXJ74_RS17570 (KXJ74_17570)	28195..28899	+	705	Protein_27	IS6-like element IS1006 family transposase	-
KXJ74_RS18440	29319..30341	+	1023	WP_228738035.1	hypothetical protein	-
KXJ74_RS17580 (KXJ74_17580)	30646..30966	+	321	WP_219191782.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KXJ74_RS17585 (KXJ74_17585)	30959..31231	+	273	WP_219191783.1	NadS family protein	Antitoxin
KXJ74_RS17590 (KXJ74_17590)	31312..31818	-	507	WP_005254456.1	hypothetical protein	-
KXJ74_RS18445	31837..32103	-	267	WP_005254455.1	hypothetical protein	-
KXJ74_RS17595 (KXJ74_17595)	32104..32535	-	432	WP_005254452.1	hypothetical protein	-
KXJ74_RS17600 (KXJ74_17600)	32551..33480	-	930	WP_219191784.1	hypothetical protein	-
KXJ74_RS17605 (KXJ74_17605)	33702..33947	+	246	WP_000246756.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
KXJ74_RS17610 (KXJ74_17610)	33937..34230	+	294	WP_000221358.1	type II toxin-antitoxin system RelE/ParE family toxin	-
KXJ74_RS17615 (KXJ74_17615)	34323..34625	+	303	WP_136144098.1	hypothetical protein	-
KXJ74_RS17620 (KXJ74_17620)	34771..35376	-	606	WP_087696031.1	alpha/beta hydrolase	-
KXJ74_RS17625 (KXJ74_17625)	35690..35977	+	288	WP_219191785.1	BrnT family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3'')-Ib / aph(6)-Id / tet(A)	-	1..71994	71994

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12192.97 Da
• Isoelectric Point: 7.9849

>T209893 WP_219191782.1 NZ_CP079749:30646-30966 [Acinetobacter johnsonii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVVYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T209893 NZ_CP104330:1956253-1956356 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10177.62 Da
• Isoelectric Point: 8.6793

>AT209893 WP_219191783.1 NZ_CP079749:30959-31231 [Acinetobacter johnsonii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIREVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT209893 NZ_CP104330:c1956394-1956123 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT