Detailed information of TA system TA209888

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3515157..3515851
• Replicon: chromosome
• Accession: NZ_CP079747
• Organism: Escherichia coli strain DL21

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: KXJ73_RS17205
• Protein ID: WP_001263493.1
• Coordinates: 3515157..3515555 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: KXJ73_RS17210
• Protein ID: WP_000554757.1
• Coordinates: 3515558..3515851 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3510817)	3510817..3510897	-	81	NuclAT_13	-	-
- (3510817)	3510817..3510897	-	81	NuclAT_13	-	-
- (3510817)	3510817..3510897	-	81	NuclAT_13	-	-
- (3510817)	3510817..3510897	-	81	NuclAT_13	-	-
KXJ73_RS17175 (3510157)	3510157..3511401	-	1245	WP_000189541.1	esterase FrsA	-
KXJ73_RS17180 (3511493)	3511493..3511951	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
KXJ73_RS17185 (3512212)	3512212..3513669	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
KXJ73_RS17190 (3513726)	3513726..3514247	-	522	Protein_3369	peptide chain release factor H	-
KXJ73_RS17195 (3514246)	3514246..3514449	-	204	Protein_3370	RtcB family protein	-
KXJ73_RS17200 (3514695)	3514695..3515147	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
KXJ73_RS17205 (3515157)	3515157..3515555	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KXJ73_RS17210 (3515558)	3515558..3515851	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KXJ73_RS17215 (3515903)	3515903..3516958	-	1056	WP_001226164.1	DNA polymerase IV	-
KXJ73_RS17220 (3517029)	3517029..3517814	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
KXJ73_RS17225 (3517786)	3517786..3519498	+	1713	Protein_3376	flagellar biosynthesis protein FlhA	-
KXJ73_RS17230 (3519714)	3519714..3520211	-	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T209888 WP_001263493.1 NZ_CP079747:c3515555-3515157 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T209888 NZ_CP104330:c1108315-1107986 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT209888 WP_000554757.1 NZ_CP079747:c3515851-3515558 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT209888 NZ_CP104330:c1108653-1108336 [Escherichia coli]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6