Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3927509..3928106 Replicon chromosome
Accession NZ_CP079725
Organism Klebsiella pneumoniae strain KP3295

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KXI30_RS18975 Protein ID WP_004142563.1
Coordinates 3927789..3928106 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KXI30_RS18970 Protein ID WP_004142561.1
Coordinates 3927509..3927796 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KXI30_RS18940 (3923589) 3923589..3923837 + 249 WP_020325243.1 DUF1158 domain-containing protein -
KXI30_RS18945 (3923855) 3923855..3924196 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KXI30_RS18950 (3924227) 3924227..3925342 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
KXI30_RS18955 (3925522) 3925522..3926103 + 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
KXI30_RS18960 (3926103) 3926103..3926471 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
KXI30_RS18965 (3926591) 3926591..3927244 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
KXI30_RS18970 (3927509) 3927509..3927796 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KXI30_RS18975 (3927789) 3927789..3928106 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KXI30_RS18980 (3928291) 3928291..3929334 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
KXI30_RS18985 (3930004) 3930004..3930870 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
KXI30_RS18990 (3930979) 3930979..3932406 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T209799 WP_004142563.1 NZ_CP079725:c3928106-3927789 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T209799 NZ_CP104310:77490-77795 [Escherichia coli O157:H7]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT209799 WP_004142561.1 NZ_CP079725:c3927796-3927509 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT209799 NZ_CP104310:77270-77488 [Escherichia coli O157:H7]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References