Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 344019..344605 Replicon chromosome
Accession NZ_CP079725
Organism Klebsiella pneumoniae strain KP3295

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag KXI30_RS01585 Protein ID WP_002920800.1
Coordinates 344237..344605 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag KXI30_RS01580 Protein ID WP_004174006.1
Coordinates 344019..344240 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KXI30_RS01560 (340176) 340176..341102 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KXI30_RS01565 (341099) 341099..342376 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
KXI30_RS01570 (342373) 342373..343140 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
KXI30_RS01575 (343142) 343142..343855 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
KXI30_RS01580 (344019) 344019..344240 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KXI30_RS01585 (344237) 344237..344605 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KXI30_RS01590 (344878) 344878..346194 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
KXI30_RS01595 (346301) 346301..347188 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
KXI30_RS01600 (347185) 347185..348030 + 846 WP_004181473.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
KXI30_RS01605 (348032) 348032..349102 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 341099..349839 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T209787 WP_002920800.1 NZ_CP079725:344237-344605 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T209787 NZ_CP104309:2896950-2897057 [Escherichia coli O157:H7]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT209787 WP_004174006.1 NZ_CP079725:344019-344240 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT209787 NZ_CP104309:c2896902-2896843 [Escherichia coli O157:H7]
GTCTGGTTTCAAGATTAGCCCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References