Detailed information of TA system TA209741

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5253704..5254299
• Replicon: chromosome
• Accession: NZ_CP079712
• Organism: Pseudomonas aeruginosa strain PAO1-UW

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: KXE08_RS24335
• Protein ID: WP_003113526.1
• Coordinates: 5254021..5254299 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KXE08_RS24330
• Protein ID: WP_003113527.1
• Coordinates: 5253704..5254009 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXE08_RS24300 (KXE08_24225)	5248817..5249107	-	291	WP_033992920.1	DUF5447 family protein	-
KXE08_RS24305 (KXE08_24230)	5249319..5249591	-	273	WP_003115921.1	hypothetical protein	-
KXE08_RS24310	5249717..5249986	+	270	WP_071536122.1	hypothetical protein	-
KXE08_RS24315 (KXE08_24235)	5250121..5251218	+	1098	WP_169917322.1	protein phosphatase 2C domain-containing protein	-
KXE08_RS24320 (KXE08_24240)	5251221..5252270	+	1050	WP_003113529.1	serine/threonine-protein kinase	-
KXE08_RS24325 (KXE08_24245)	5252339..5253340	+	1002	WP_228772168.1	serine/threonine-protein kinase	-
KXE08_RS24330 (KXE08_24250)	5253704..5254009	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
KXE08_RS24335 (KXE08_24255)	5254021..5254299	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KXE08_RS24340	5254352..5254480	-	129	Protein_4806	integrase	-
KXE08_RS24345 (KXE08_24260)	5254628..5256856	+	2229	WP_003113525.1	TonB-dependent receptor	-
KXE08_RS24350 (KXE08_24265)	5256926..5257573	-	648	WP_003095021.1	carbonate dehydratase	-
KXE08_RS24355 (KXE08_24270)	5257635..5258873	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T209741 WP_003113526.1 NZ_CP079712:c5254299-5254021 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T209741 NZ_CP104292:c107737-106415 [Stenotrophomonas maltophilia]
ATGGGTACGCTGCAGGTCTGGATGAATGGTGTGGAAGTCGCACTTTGGGATGATGTCCGGAACGGGTCATCCCGGTTGTC
GTATGTCAGCCAGTGGATCAGCTCGCCCCAGTCCCGACCGTTGTCGCTCTCTCTACCCCTGCTCCCAGATGGCGAGAGCC
ATCGGGGCAACGTCGTCAACGACTACTTCGATAACCTTCTTCCCGATAACGTCGCCATACGCAACCGGATCCGCGACCGG
TTCGCCACGCGCGGCAGCGATACGTTCGATCTTCTTGAAGCCATTGGACGGGATTGTGTCGGCGCAGTGCAGCTGTTGCC
GCCTGCGCATCCACCCGAAGACATCCAGCAGATCACGTGCGAGGTGTTGGACGAAGCAGGTGTCGCTACGATCCTGCGTC
AGGTGACGACAGGGAACCTGCCTGGCGCACCCAGCCGTGATGATGCATTCCGCATTTCCATCGCCGGTGCGCAGGAAAAG
ACCGGTCTTCTGCTGCATGCTGGCCAATGGTGCATTCCTACCGGCAACACACCATCCACTCACATCTTCAAGCTGCCGCT
TGGCATTGTTGGCAACATGCGGGCAGACATGCATCAGTCCGTGGAGAATGAATGGCTGTGCATGCATCTGCTTTCCGCCT
TTGATATCCCCGTGGCCGAGACGCAGATGGGCCGCTTCAACGACCAACGTGCTCTCATTGTCGAGCGGTTTGACCGTCGC
CTGTCTGCCGACCGCCGGTGGTGGCTGCGACTGCCACAGGAAGACATGTGTCAGGTCTTTGGTCTGCCGGCTGCCAGAAA
GTATGAATCCGATGGCGGCCCCGGTATCGTTCCGATCATGGATCTGCTTCGACAGTCCGAACACCCCGAGGATCGCGCGA
TCTTCTTCAAGACGCAGATTCTTTTCTGGATGCTGGCCGCAACCGACGGCCATGCCAAGAATTTCAGTGTTCACCTGGAA
GCCGGTGGACGCTTCCGCCTGACGCCCGTCTACGACGTGCTGTCGGTATATCCCATCCTCGGCTGGGGACCCAATCAACT
CGATCCACGAAACGCACAGCTCGCCATGGCCGTACATGGCAAGAACCGCCACCGGAAACTGCACCTTGTGCGACGGCGGC
ACTGGAATGAAACCGCGCGCGCCTGCGGCGTTGCCGATGGTGCAGAACCATGGATCAAGACATTGATCGCGGCCGTCGAG
CCCGCCATCCAGCAGGTCACCCGTCAGCTCCCGGATGACTTCCCCTCAAACGTGTCCGAACCGATTTTCAACGGACTTCG
TGCTGCGGCAGCACGCCTTTCCAGCATGGCCCCCGAGGCTTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT209741 WP_003113527.1 NZ_CP079712:c5254009-5253704 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT209741 NZ_CP104292:c107998-107741 [Stenotrophomonas maltophilia]
ATGGACAGGCCGCTGCACACAGTGAGGACAGCCCAACAACTTGCGCCCCTGATGCGTGCTTTCCGGCGCCAGGCGGGCCT
GAGCCAGGCTCAGCTGGCGGAGCGACTCGGGATCAGCCGCCAGGCAGTGGGCGCGCTGGAACGCGACCCTGCATCGGCAA
GTTTTGAACGCCTGATGCGGATCTGGGCGGTGCTGGGTCTGGAGGTCAGCCTCCAGCAACGAAGTCCCCAGGAGAACACG
TCGTCATTGGAGTGGTGA

Structures

Toxin

• AlphaFold DB: V6ALY3