Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3944507..3945104 Replicon chromosome
Accession NZ_CP079683
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC30_AI1547MPCH

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KW563_RS19380 Protein ID WP_004142563.1
Coordinates 3944787..3945104 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KW563_RS19375 Protein ID WP_004142561.1
Coordinates 3944507..3944794 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW563_RS19345 (3940602) 3940602..3940850 + 249 WP_002893037.1 DUF1158 domain-containing protein -
KW563_RS19350 (3940868) 3940868..3941209 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KW563_RS19355 (3941240) 3941240..3942355 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
KW563_RS19360 (3942535) 3942535..3943119 + 585 WP_071579668.1 TetR/AcrR family transcriptional regulator -
KW563_RS19365 (3943116) 3943116..3943484 + 369 WP_040171587.1 MmcQ/YjbR family DNA-binding protein -
KW563_RS19370 (3943604) 3943604..3944242 + 639 WP_064277739.1 oxygen-insensitive NAD(P)H nitroreductase -
KW563_RS19375 (3944507) 3944507..3944794 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KW563_RS19380 (3944787) 3944787..3945104 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KW563_RS19385 (3945289) 3945289..3946332 - 1044 WP_040153598.1 DUF2157 domain-containing protein -
KW563_RS19390 (3947002) 3947002..3947868 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
KW563_RS19395 (3947977) 3947977..3949404 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T209708 WP_004142563.1 NZ_CP079683:c3945104-3944787 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T209708 NZ_CP104284:c299091-298789 [Enterobacter hormaechei subsp. steigerwaltii]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGACAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGGATCTATTTTAAGGACCAGGGCAATAGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT209708 WP_004142561.1 NZ_CP079683:c3944794-3944507 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT209708 NZ_CP104284:c298782-298495 [Enterobacter hormaechei subsp. steigerwaltii]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGCTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References