Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3494080..3494677 Replicon chromosome
Accession NZ_CP079673
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC29_CMC309MPCH

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KW562_RS16715 Protein ID WP_004142563.1
Coordinates 3494080..3494397 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KW562_RS16720 Protein ID WP_004142561.1
Coordinates 3494390..3494677 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW562_RS16700 (3489783) 3489783..3491210 - 1428 WP_004176980.1 MFS transporter -
KW562_RS16705 (3491319) 3491319..3492185 + 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
KW562_RS16710 (3492852) 3492852..3493895 + 1044 WP_004178895.1 DUF2157 domain-containing protein -
KW562_RS16715 (3494080) 3494080..3494397 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KW562_RS16720 (3494390) 3494390..3494677 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KW562_RS16725 (3494945) 3494945..3495595 - 651 Protein_3262 oxygen-insensitive NAD(P)H nitroreductase -
KW562_RS16730 (3495715) 3495715..3496083 - 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
KW562_RS16735 (3496083) 3496083..3496664 - 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
KW562_RS16740 (3496844) 3496844..3497959 + 1116 WP_020316605.1 MBL fold metallo-hydrolase -
KW562_RS16745 (3497990) 3497990..3498331 + 342 WP_166689814.1 RamA family antibiotic efflux transcriptional regulator -
KW562_RS16750 (3498349) 3498349..3498597 - 249 WP_002893037.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T209694 WP_004142563.1 NZ_CP079673:3494080-3494397 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T209694 NZ_CP104274:2754120-2754227 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT209694 WP_004142561.1 NZ_CP079673:3494390-3494677 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT209694 NZ_CP104274:c2754067-2754006 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References