Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4066856..4067453 Replicon chromosome
Accession NZ_CP079668
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC28_CMC387MPCH

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KW561_RS19865 Protein ID WP_004142563.1
Coordinates 4067136..4067453 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KW561_RS19860 Protein ID WP_004142561.1
Coordinates 4066856..4067143 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW561_RS19830 (4062936) 4062936..4063184 + 249 WP_002893037.1 DUF1158 domain-containing protein -
KW561_RS19835 (4063202) 4063202..4063543 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KW561_RS19840 (4063574) 4063574..4064689 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
KW561_RS19845 (4064869) 4064869..4065450 + 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
KW561_RS19850 (4065450) 4065450..4065818 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
KW561_RS19855 (4065938) 4065938..4066588 + 651 Protein_3913 oxygen-insensitive NAD(P)H nitroreductase -
KW561_RS19860 (4066856) 4066856..4067143 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KW561_RS19865 (4067136) 4067136..4067453 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KW561_RS19870 (4067638) 4067638..4068681 - 1044 WP_004178895.1 DUF2157 domain-containing protein -
KW561_RS19875 (4069348) 4069348..4070214 - 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
KW561_RS19880 (4070323) 4070323..4071750 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T209669 WP_004142563.1 NZ_CP079668:c4067453-4067136 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T209669 NZ_CP104209:2560016-2560300 [Empedobacter stercoris]
ATGGAAACATCAAAATTTGATATAGCCGATTATTTAGATAGTAATGAAATGATTGCTGAATACTTAAATGCAGTCTTAGA
AGATGGTAATGAAAATGAAATCATCACTGCTATTGGAAACATTGCAAAAGCAATTGGAATGACTAAAATAGCAGAAGAAA
CAGGTCTTAGTCGTCCAAGTTTATATAAAGCTTTATCTGATGGTTCAAAACCTCAATTTGGAACTATAATGAAAGTTTTA
AAAGCTGTGGGAGGACAAATACAAGTAAATCCTGTTCAAGCTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT209669 WP_004142561.1 NZ_CP079668:c4067143-4066856 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT209669 NZ_CP104209:2559710-2559868 [Empedobacter stercoris]
ATGTACTTTATTGAAAAAACTACTGATTTTGATAAATGGTTTCGTAAGTTAAAAGACTTACGAGCAAAGGCTAAAATTTT
AGTTAGAATTCAAAAATTAGAAAAAGAAGAACACTTTGGAGATTGTGAACCTGTTGGCAATGGAATTCGAGAATTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References