Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4022245..4022842 Replicon chromosome
Accession NZ_CP079655
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC27_S471MPC

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KW560_RS19855 Protein ID WP_004142563.1
Coordinates 4022525..4022842 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KW560_RS19850 Protein ID WP_004142561.1
Coordinates 4022245..4022532 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW560_RS19820 (4018340) 4018340..4018588 + 249 WP_002893037.1 DUF1158 domain-containing protein -
KW560_RS19825 (4018606) 4018606..4018947 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KW560_RS19830 (4018978) 4018978..4020093 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
KW560_RS19835 (4020273) 4020273..4020857 + 585 WP_071579668.1 TetR/AcrR family transcriptional regulator -
KW560_RS19840 (4020854) 4020854..4021222 + 369 WP_040171587.1 MmcQ/YjbR family DNA-binding protein -
KW560_RS19845 (4021342) 4021342..4021980 + 639 WP_064277739.1 oxygen-insensitive NAD(P)H nitroreductase -
KW560_RS19850 (4022245) 4022245..4022532 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KW560_RS19855 (4022525) 4022525..4022842 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KW560_RS19860 (4023027) 4023027..4024070 - 1044 WP_040153598.1 DUF2157 domain-containing protein -
KW560_RS19865 (4024740) 4024740..4025606 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
KW560_RS19870 (4025715) 4025715..4027142 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T209650 WP_004142563.1 NZ_CP079655:c4022842-4022525 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T209650 NZ_CP104206:c2017336-2017229 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT209650 WP_004142561.1 NZ_CP079655:c4022532-4022245 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT209650 NZ_CP104206:2017383-2017449 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References