Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-YefM
Location 1478535..1479037 Replicon chromosome
Accession NC_004757
Organism Nitrosomonas europaea ATCC 19718

Toxin (Protein)


Gene name yoeB Uniprot ID Q82UW6
Locus tag ALW85_RS07055 Protein ID WP_011111929.1
Coordinates 1478535..1478795 (-) Length 87 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A837X5B7
Locus tag ALW85_RS07060 Protein ID WP_041356748.1
Coordinates 1478795..1479037 (-) Length 81 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ALW85_RS07020 (NE1345) 1474137..1474397 - 261 WP_011111923.1 Txe/YoeB family addiction module toxin -
ALW85_RS07025 (NE1346) 1474400..1474660 - 261 WP_041356746.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
ALW85_RS07030 (NE1347) 1474936..1475978 + 1043 Protein_1357 IS481 family transposase -
ALW85_RS07035 (NE1349) 1476105..1476500 - 396 WP_011111927.1 type II toxin-antitoxin system VapC family toxin -
ALW85_RS07040 (NE1350) 1476508..1476753 - 246 WP_011111928.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
ALW85_RS07050 (NE1351) 1477435..1478478 + 1044 WP_011110809.1 IS481 family transposase -
ALW85_RS07055 (NE1352) 1478535..1478795 - 261 WP_011111929.1 Txe/YoeB family addiction module toxin Toxin
ALW85_RS07060 (RK28_RS07055) 1478795..1479037 - 243 WP_041356748.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
ALW85_RS07065 (RK28_RS07060) 1479355..1479621 + 267 WP_041357169.1 hypothetical protein -
ALW85_RS07070 (NE1353) 1479618..1479917 + 300 WP_011111930.1 type II toxin-antitoxin system RelE/ParE family toxin -
ALW85_RS07075 (NE1354) 1480079..1480357 - 279 Protein_1365 XRE family transcriptional regulator -
ALW85_RS14305 1480354..1480452 - 99 WP_198407841.1 type II toxin-antitoxin system RelE/ParE family toxin -
ALW85_RS14310 1480424..1480645 - 222 WP_041356749.1 DUF2442 domain-containing protein -
ALW85_RS07085 (RK28_RS07080) 1480651..1480809 - 159 WP_198407842.1 DUF4160 domain-containing protein -
ALW85_RS07090 (NE1355) 1480914..1481234 - 321 WP_011111932.1 putative addiction module antidote protein -
ALW85_RS07095 (NE1356) 1481227..1481535 - 309 WP_041357171.1 type II toxin-antitoxin system RelE/ParE family toxin -
ALW85_RS07105 (NE1358) 1482114..1482425 + 312 WP_198407843.1 HigA family addiction module antidote protein -
ALW85_RS07110 (NE1360) 1482500..1483081 - 582 WP_041356750.1 RloB family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1475610..1500344 24734


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-85)

Antitoxin

(1-69)


Sequences


Toxin        


Download         Length: 87 a.a.        Molecular weight: 10118.77 Da        Isoelectric Point: 10.1619

>T20965 WP_011111929.1 NC_004757:c1478795-1478535 [Nitrosomonas europaea ATCC 19718]
MWELRYTHQAQKDAKKLASSGLKDKAEELLAVVRNNPYQTPPPYEKLVGDLAGACSRRINIQHRLVYQVLERERIVKVLR
MWTHYV

Download         Length: 261 bp

>T20965 NC_004757:c1478795-1478535 [Nitrosomonas europaea ATCC 19718]
ATGTGGGAGCTGCGATATACCCATCAAGCGCAAAAGGATGCAAAAAAGCTGGCATCGTCTGGGCTTAAAGATAAGGCAGA
GGAGTTGTTAGCGGTTGTGAGGAATAATCCGTACCAAACCCCACCCCCCTATGAAAAGCTGGTTGGTGATTTGGCTGGAG
CCTGTTCACGCCGTATCAACATCCAGCACAGGCTCGTGTATCAGGTGTTGGAGCGGGAGAGGATAGTAAAGGTTTTGCGT
ATGTGGACTCATTATGTGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9010.25 Da        Isoelectric Point: 4.2340

>AT20965 WP_041356748.1 NC_004757:c1479037-1478795 [Nitrosomonas europaea ATCC 19718]
MAILNATEARARLYALIDEAAETHQPIVIKGKRSSAVLLSEEDWNAINETLYLVSIPGMRESIMEGMKTDVDECSRELDW

Download         Length: 243 bp

>AT20965 NC_004757:c1479037-1478795 [Nitrosomonas europaea ATCC 19718]
ATGGCAATACTAAACGCAACAGAAGCAAGAGCGAGGTTGTACGCCTTAATTGATGAGGCCGCAGAAACACACCAGCCTAT
TGTGATTAAGGGAAAAAGGTCAAGTGCGGTGCTTTTATCGGAGGAAGACTGGAACGCTATCAACGAGACGCTTTACTTGG
TTTCTATCCCGGGAATGCGCGAATCCATTATGGAGGGTATGAAAACTGATGTGGATGAGTGCAGTAGGGAATTGGATTGG
TAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB Q82UW6


Antitoxin

Source ID Structure
AlphaFold DB A0A837X5B7

References