Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2104616..2105213 Replicon chromosome
Accession NZ_CP079645
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC24_S468MC

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KW559_RS10295 Protein ID WP_004142563.1
Coordinates 2104616..2104933 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KW559_RS10300 Protein ID WP_004142561.1
Coordinates 2104926..2105213 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW559_RS10280 (2100316) 2100316..2101743 - 1428 WP_009308097.1 MFS transporter -
KW559_RS10285 (2101852) 2101852..2102718 + 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
KW559_RS10290 (2103388) 2103388..2104431 + 1044 WP_040153598.1 DUF2157 domain-containing protein -
KW559_RS10295 (2104616) 2104616..2104933 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KW559_RS10300 (2104926) 2104926..2105213 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KW559_RS10305 (2105478) 2105478..2106116 - 639 WP_064277739.1 oxygen-insensitive NAD(P)H nitroreductase -
KW559_RS10310 (2106236) 2106236..2106604 - 369 WP_040171587.1 MmcQ/YjbR family DNA-binding protein -
KW559_RS10315 (2106601) 2106601..2107185 - 585 WP_071579668.1 TetR/AcrR family transcriptional regulator -
KW559_RS10320 (2107365) 2107365..2108480 + 1116 WP_012737592.1 MBL fold metallo-hydrolase -
KW559_RS10325 (2108511) 2108511..2108852 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KW559_RS10330 (2108870) 2108870..2109118 - 249 WP_002893037.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T209622 WP_004142563.1 NZ_CP079645:2104616-2104933 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T209622 NZ_CP104174:556362-556766 [Bradyrhizobium sp. CB1015]
GTGAGCGCGTTCTTCGACAGCAATATCTTGATCTATGCCTATTCGACCGACGCCCGGCGAGGACCGGCGTTGAACGCCCT
TGCCGGAGGCGGCGTGATCAGTGCGCAGGTGTTGAACGAGTTCACCAACGTGCTGCGCAAGAAGCAGCAGCAGGACTGGC
CGGTCATTGAGGCAGCCGTCCAGACGATCCGGTTTCGCTTTCCGGACATCGTGCCTCTGACCGCGGATACGCACGCAGCT
GCGATTGCGCTCGCCCGCGATCATTCCGTAGCGTTCTACGACGCCCTCATCATCGCGTCCGCGATCGAGACCGGATGCGA
TACGCTTTATAGCGAAGACCTCCAGCACAGGCGCAGCATCGGCGGGCTCACCATCCTCAATCCGTTCCTCGGCATGACGC
CGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT209622 WP_004142561.1 NZ_CP079645:2104926-2105213 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT209622 NZ_CP104174:556135-556365 [Bradyrhizobium sp. CB1015]
GTGCTGGTTTCCAAATGGGGCAACAGCCTTGCAGTGCGCCTGCCCAAAGCGCTGGTCGAAGCCTTGAAGCTCTCGCCTGG
TGACGAACTCAACGTGGTCAAAGCCTCGAAGGGCGAGATTGCGGTGGAGAAGGTCGACAAACGAGCGGAGTTTCTCAAGC
AGGTGGAGCAATTCCATTTTCCGCTCCCCGAAGGCTATAAATTCGATCGGGACGAGGCGAACGAACGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References