Detailed information of TA system TA020960

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1200249..1200899
• Replicon: chromosome
• Accession: NC_004757
• Organism: Nitrosomonas europaea ATCC 19718

Toxin (Protein)

• Gene name: graT
• Uniprot ID: Q82VH9
• Locus tag: ALW85_RS05760
• Protein ID: WP_011111702.1
• Coordinates: 1200249..1200530 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ALW85_RS05765
• Protein ID: WP_050731300.1
• Coordinates: 1200573..1200899 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ALW85_RS05730 (NE1103)	1195700..1196194	-	495	WP_011111700.1	GNAT family N-acetyltransferase	-
ALW85_RS05735 (NE1104)	1196191..1196463	-	273	WP_011111701.1	DUF1778 domain-containing protein	-
ALW85_RS05740 (NE1105)	1196708..1198576	+	1869	WP_050731249.1	TonB-dependent receptor	-
ALW85_RS13940	1198601..1199376	+	776	Protein_1113	IS5 family transposase	-
ALW85_RS05755 (NE1108)	1199381..1200004	+	624	Protein_1114	TonB-dependent receptor	-
ALW85_RS05760 (NE1109)	1200249..1200530	+	282	WP_011111702.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ALW85_RS05765 (NE1110)	1200573..1200899	+	327	WP_050731300.1	HigA family addiction module antidote protein	Antitoxin
ALW85_RS05770 (NE1111)	1200892..1202304	-	1413	WP_011111704.1	efflux transporter outer membrane subunit	-
ALW85_RS05775 (NE1112)	1202320..1205421	-	3102	WP_011111705.1	multidrug efflux RND transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1199008..1199376	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (27-74)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11038.72 Da
• Isoelectric Point: 10.3438

>T20960 WP_011111702.1 NC_004757:1200249-1200530 [Nitrosomonas europaea ATCC 19718]
MIKTFATKETAALFANEKIRRLPPEILRVARRKMAQLHRVSSIEELRIPPGNRLEKLSGNRNEQWSIRINDQWRICFRFE
AGDVFDVEITDYH

Nucleotide

• Length: 282 bp

>T20960 NC_004757:1200249-1200530 [Nitrosomonas europaea ATCC 19718]
ATGATCAAAACTTTCGCCACGAAAGAAACAGCTGCTTTATTTGCTAATGAAAAGATCAGGCGACTGCCACCAGAAATTCT
CCGGGTTGCTCGTCGAAAAATGGCGCAGTTACATCGTGTTTCATCAATTGAGGAACTGCGTATCCCACCTGGAAACAGGC
TTGAAAAATTGTCTGGTAACCGGAATGAACAATGGAGCATTCGCATCAACGATCAATGGCGAATTTGTTTCCGATTCGAA
GCAGGCGATGTATTTGATGTCGAGATTACCGATTATCATTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12374.25 Da
• Isoelectric Point: 6.8805

>AT20960 WP_050731300.1 NC_004757:1200573-1200899 [Nitrosomonas europaea ATCC 19718]
MDFSDVAEGGKLHPIHPGEILREEFLMPLKITPHALSLALQIPATRINDIVRERRAITTDTALRLARYFGNTAEFWMGLQ
IDYDMTITRDSLRGALNRIQRFEPTHIS

Nucleotide

• Length: 327 bp

>AT20960 NC_004757:1200573-1200899 [Nitrosomonas europaea ATCC 19718]
ATGGATTTCAGTGATGTGGCTGAAGGTGGAAAATTGCACCCCATTCACCCTGGCGAGATTCTGCGCGAAGAATTTCTGAT
GCCGTTAAAAATCACTCCTCATGCACTTTCTTTGGCATTGCAAATTCCGGCCACCCGTATCAACGATATCGTACGGGAGC
GTCGTGCAATTACTACCGACACCGCCCTCAGACTGGCGCGGTATTTTGGTAATACCGCTGAATTCTGGATGGGTCTTCAA
ATCGATTACGATATGACTATTACCCGCGATTCCTTACGTGGGGCATTGAATAGAATTCAACGATTTGAACCTACCCATAT
CAGTTAA

Structures

Toxin

• AlphaFold DB: Q82VH9