Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4098537..4099134 Replicon chromosome
Accession NZ_CP079629
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC18_CMC307MC

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KW554_RS20230 Protein ID WP_004142563.1
Coordinates 4098817..4099134 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KW554_RS20225 Protein ID WP_004142561.1
Coordinates 4098537..4098824 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW554_RS20195 (4094617) 4094617..4094865 + 249 WP_002893037.1 DUF1158 domain-containing protein -
KW554_RS20200 (4094883) 4094883..4095224 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KW554_RS20205 (4095255) 4095255..4096370 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
KW554_RS20210 (4096550) 4096550..4097131 + 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
KW554_RS20215 (4097131) 4097131..4097499 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
KW554_RS20220 (4097619) 4097619..4098269 + 651 Protein_3989 oxygen-insensitive NAD(P)H nitroreductase -
KW554_RS20225 (4098537) 4098537..4098824 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KW554_RS20230 (4098817) 4098817..4099134 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KW554_RS20235 (4099319) 4099319..4100362 - 1044 WP_004178895.1 DUF2157 domain-containing protein -
KW554_RS20240 (4101029) 4101029..4101895 - 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
KW554_RS20245 (4102004) 4102004..4103431 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T209557 WP_004142563.1 NZ_CP079629:c4099134-4098817 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T209557 NZ_CP104132:368207-368626 [Sinorhizobium sp. K101]
ATGATTGTCCTCGATACGAATGTCGTCTCCGAGGTGATGAAACCGGCCGCCGATCCGGCCGTGCGCTCCTGGTTGAACGA
GCAGGTCGCTGAAACCCTTTATCTGGCCAGTGTGACCTTGGCCGAACTGCTGTTCGGCATCGGTGCGCTGCCGGATGGTC
GGCGCAAGAAGGCTCTGGCCGAAATGTTTGACGGCGTGCTCGAACTGTTTGGCGATCGCGTATTGCCGTTCGATATCGGA
GCTGCTCGCTACTATGCCGGGCTTGCCGTGACCGCTCGAGCCGCCGGCAAGGGATTTCCGACGCCCGACGGATATATTGC
CGCCATTGCCGCCTCCAGGGGATTCCTCGTCGCGACGCGCGATATCAGCCCGTTCGAAGCGGCTGGACTTACCGTGGTAA
ATCCTTGGCACCACCGATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT209557 WP_004142561.1 NZ_CP079629:c4098824-4098537 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT209557 NZ_CP104132:367956-368210 [Sinorhizobium sp. K101]
ATGGCGATCCTGACGGTTCGAAATGTGCCCGATGAAGTGCATCGCGCATTGCGCGTGAGAGCGGCGATGCACGGCCGCAG
CACCGAGGCGGAAGTGCGGGAGATTCTGGAAAGTGCGGTGAAGCCCGAGCAGCGCGTTCGCATGGGCGATGCCCTGGCCG
AGCTCGGCCGGCGATTGGGCCTGACCAATGACGATTTCGCAGTGTTCGACGAGATACGTGACAAGGCGCCGGCGCAGCCG
ATGAGATTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References