Detailed information of TA system TA209544

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 13599..14256
• Replicon: plasmid pCMC307P_P2
• Accession: NZ_CP079626
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC17_CMC307PC

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: KW552_RS28075
• Protein ID: WP_000270043.1
• Coordinates: 13906..14256 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KW552_RS28070
• Protein ID: WP_000124640.1
• Coordinates: 13599..13901 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW552_RS28020 (KW552_28005)	9233..9661	+	429	WP_000591076.1	hypothetical protein	-
KW552_RS28025 (KW552_28010)	9719..10078	+	360	WP_000422769.1	hypothetical protein	-
KW552_RS28030 (KW552_28015)	10078..10524	+	447	WP_000919343.1	hypothetical protein	-
KW552_RS28035 (KW552_28020)	10521..11039	+	519	WP_000210757.1	nitrite reductase	-
KW552_RS28040 (KW552_28025)	11039..11269	+	231	WP_000972665.1	hypothetical protein	-
KW552_RS28045 (KW552_28030)	11256..12113	+	858	WP_001167036.1	hypothetical protein	-
KW552_RS29800	12139..12330	+	192	WP_001270409.1	hypothetical protein	-
KW552_RS28055 (KW552_28040)	12333..12860	+	528	WP_001210659.1	thermonuclease family protein	-
KW552_RS28060 (KW552_28045)	12918..13190	+	273	WP_001043046.1	HU family DNA-binding protein	-
KW552_RS28065 (KW552_28050)	13279..13572	+	294	WP_001239998.1	hypothetical protein	-
KW552_RS28070 (KW552_28055)	13599..13901	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
KW552_RS28075 (KW552_28060)	13906..14256	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KW552_RS28080 (KW552_28065)	14419..14967	+	549	WP_001061573.1	hypothetical protein	-
KW552_RS28085 (KW552_28070)	15308..15502	+	195	WP_000343597.1	hypothetical protein	-
KW552_RS28090 (KW552_28075)	15513..15884	+	372	WP_000516918.1	hypothetical protein	-
KW552_RS28095 (KW552_28080)	15877..16347	+	471	WP_001281821.1	hypothetical protein	-
KW552_RS28100 (KW552_28085)	16362..16697	-	336	WP_000683477.1	hypothetical protein	-
KW552_RS28105 (KW552_28090)	16794..17282	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
KW552_RS28110 (KW552_28095)	17285..17782	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ARR-3 / cmlA1 / blaOXA-10 / ant(3'')-Ia / qacE / sul1 / armA / msr(E) / mph(E) / blaCTX-M-15 / aac(6')-Ib / blaOXA-9 / blaTEM-1A	-	1..197458	197458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T209544 WP_000270043.1 NZ_CP079626:c14256-13906 [Klebsiella pneumoniae subsp. pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T209544 NZ_CP104128:587820-588239 [Sinorhizobium sp. M103]
ATGATTGTCCTCGATACGAATGTCGTCTCCGAGGTGATGAAACCGGCCGCCGATCCGGCCGTGCGCTCCTGGTTGAACGA
GCAGGTCGCTGAAACCCTTTATCTGGCCAGTGTGACCTTGGCCGAACTGCTGTTCGGCATCGGTGCGCTGCCGGATGGTC
GGCGCAAGAAGGCTCTGGCCGAAATGTTTGACGGCGTGCTCGAACTGTTTGGCGATCGCGTATTGCCGTTCGATATCGGA
GCTGCTCGCTACTATGCCGGGCTTGCCGTGACCGCTCGAGCCGCCGGCAAGGGATTTCCGACGCCCGACGGATATATTGC
CGCCATTGCCGCCTCCAGGGGATTCCTCGTCGCGACGCGCGATATCAGCCCGTTCGAAGCGGCTGGACTTACCGTGGTAA
ATCCTTGGCACCACCGATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT209544 WP_000124640.1 NZ_CP079626:c13901-13599 [Klebsiella pneumoniae subsp. pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT209544 NZ_CP104128:587569-587823 [Sinorhizobium sp. M103]
ATGGCGATCCTGACGGTTCGAAATGTGCCCGATGAAGTGCATCGCGCATTGCGCGTGAGAGCGGCGATGCACGGCCGCAG
CACCGAGGCGGAAGTGCGGGAGATTCTGGAAAGTGCGGTGAAGCCCGAGCAGCGCGTTCGCATGGGCGATGCCCTGGCCG
AGCTCGGCCGGCGATTGGGCCTGACCAATGACGATTTCGCAGTGTTCGACGAGATACGTGACAAGGCGCCGGCGCAGCCG
ATGAGATTTGAATGA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9