Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4113253..4113850 Replicon chromosome
Accession NZ_CP079624
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC17_CMC307PC

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KW552_RS20285 Protein ID WP_004142563.1
Coordinates 4113533..4113850 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KW552_RS20280 Protein ID WP_004142561.1
Coordinates 4113253..4113540 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW552_RS20250 (4109333) 4109333..4109581 + 249 WP_002893037.1 DUF1158 domain-containing protein -
KW552_RS20255 (4109599) 4109599..4109940 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KW552_RS20260 (4109971) 4109971..4111086 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
KW552_RS20265 (4111266) 4111266..4111847 + 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
KW552_RS20270 (4111847) 4111847..4112215 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
KW552_RS20275 (4112335) 4112335..4112985 + 651 Protein_3999 oxygen-insensitive NAD(P)H nitroreductase -
KW552_RS20280 (4113253) 4113253..4113540 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KW552_RS20285 (4113533) 4113533..4113850 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KW552_RS20290 (4114035) 4114035..4115078 - 1044 WP_004178895.1 DUF2157 domain-containing protein -
KW552_RS20295 (4115745) 4115745..4116611 - 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
KW552_RS20300 (4116720) 4116720..4118147 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T209537 WP_004142563.1 NZ_CP079624:c4113850-4113533 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T209537 NZ_CP104127:397069-397485 [Sinorhizobium sp. M103]
ATGAGCCTGTGGATGCTCGATACCAAGATTGCCGGTCATGTCATCAAGGGCGACCGCCCCGAGATCATCGATCGGTTGGT
CTCGCTTCCCATCACGGATATTGTCGTATCATCCGTCACCGAGGGGGAGTTGCTCTACGGTCTCGCCAAGCGAGGCTATC
CGAGGACGCTATCTGAACGGGTCAGGCAATTCCTTCTTCGGGTAGACGTGTTGCCCTGGAATCGCACTGCCGCCCGAAGC
TATGGCGACCTACGCGCTGCCTGTGAAGCCAAGGGCGCGGCTCTCGCGCCCCTCGATATGATGATTGCTGCTCATGCGGT
CGCGTTCGATGCGGTCCTTGTGACCCGCGACAGAGCTTTTACTCGGGTTGCGGAGCCCCTGAAGACGGACGACTGGACGG
ACCAAAAACTGAAATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT209537 WP_004142561.1 NZ_CP079624:c4113540-4113253 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT209537 NZ_CP104127:396809-397072 [Sinorhizobium sp. M103]
GTGGCAACCAAAACAGCCAAGATATTTGCCAGCGGCCGCAGCCAGGCCGTCAGGCTCCCTGCAGAGTTCCGCTTCGAGGG
CAGCGAGGTGTTCGTTCGTCGTGATCCGAAGACCGGTGATGTCATTCTGTCACGCAAGCCGGATTCATGGGACGGTCTTT
TTGAACTCTATGGCAAGGATCAGGTCGGGGACGACTTCCTGGGGCCGGCCGACCGCAAGCAGCCTTCGCAGGATAGAGAT
CCGTTCGAGGGCTGGAAGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References