Detailed information of TA system TA209525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 13599..14256
• Replicon: plasmid pAI1547M_P1
• Accession: NZ_CP079618
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC16_AI1547MC

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: KW551_RS27070
• Protein ID: WP_000270043.1
• Coordinates: 13906..14256 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KW551_RS27065
• Protein ID: WP_000124640.1
• Coordinates: 13599..13901 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW551_RS27015 (KW551_26990)	9233..9661	+	429	WP_000591076.1	hypothetical protein	-
KW551_RS27020 (KW551_26995)	9719..10078	+	360	WP_000422769.1	hypothetical protein	-
KW551_RS27025 (KW551_27000)	10078..10524	+	447	WP_000919343.1	hypothetical protein	-
KW551_RS27030 (KW551_27005)	10521..11039	+	519	WP_000210757.1	nitrite reductase	-
KW551_RS27035 (KW551_27010)	11039..11269	+	231	WP_000972665.1	hypothetical protein	-
KW551_RS27040 (KW551_27015)	11256..12113	+	858	WP_001167036.1	hypothetical protein	-
KW551_RS29185	12139..12330	+	192	WP_001270409.1	hypothetical protein	-
KW551_RS27050 (KW551_27025)	12333..12860	+	528	WP_004201083.1	thermonuclease family protein	-
KW551_RS27055 (KW551_27030)	12918..13190	+	273	WP_001043046.1	HU family DNA-binding protein	-
KW551_RS27060 (KW551_27035)	13279..13572	+	294	WP_001239998.1	hypothetical protein	-
KW551_RS27065 (KW551_27040)	13599..13901	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
KW551_RS27070 (KW551_27045)	13906..14256	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KW551_RS27075 (KW551_27050)	14419..14967	+	549	WP_001061573.1	hypothetical protein	-
KW551_RS27080 (KW551_27055)	15308..15502	+	195	WP_000343597.1	hypothetical protein	-
KW551_RS27085 (KW551_27060)	15513..15884	+	372	WP_000516918.1	hypothetical protein	-
KW551_RS27090 (KW551_27065)	15877..16347	+	471	WP_001281821.1	hypothetical protein	-
KW551_RS27095 (KW551_27070)	16362..16697	-	336	WP_000683477.1	hypothetical protein	-
KW551_RS27100 (KW551_27075)	16794..17282	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
KW551_RS27105 (KW551_27080)	17285..17782	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-4 / dfrA12 / aadA2 / qacE / sul1 / armA / msr(E) / mph(E) / blaTEM-1B	-	1..170268	170268

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T209525 WP_000270043.1 NZ_CP079618:c14256-13906 [Klebsiella pneumoniae subsp. pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T209525 NZ_CP104123:2408130-2409452 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CAGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGGCTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTTAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCTGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCGTTTTTGATGGGGTCCAGCGAGGCGCTTCGCGATCGTTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTATTGGGTGGCGCAGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTTCCGGAAGTGCAGATGCATGAAATCCTAAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGCTGCACCGTCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT209525 WP_000124640.1 NZ_CP079618:c13901-13599 [Klebsiella pneumoniae subsp. pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT209525 NZ_CP104123:2407864-2408130 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGCATTAAGCAGGCGACAATCTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACACTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9