Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4023957..4024554 Replicon chromosome
Accession NZ_CP079617
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC16_AI1547MC

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KW551_RS19875 Protein ID WP_004142563.1
Coordinates 4024237..4024554 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KW551_RS19870 Protein ID WP_004142561.1
Coordinates 4023957..4024244 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW551_RS19840 (4020052) 4020052..4020300 + 249 WP_002893037.1 DUF1158 domain-containing protein -
KW551_RS19845 (4020318) 4020318..4020659 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KW551_RS19850 (4020690) 4020690..4021805 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
KW551_RS19855 (4021985) 4021985..4022569 + 585 WP_071579668.1 TetR/AcrR family transcriptional regulator -
KW551_RS19860 (4022566) 4022566..4022934 + 369 WP_040171587.1 MmcQ/YjbR family DNA-binding protein -
KW551_RS19865 (4023054) 4023054..4023692 + 639 WP_064277739.1 oxygen-insensitive NAD(P)H nitroreductase -
KW551_RS19870 (4023957) 4023957..4024244 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KW551_RS19875 (4024237) 4024237..4024554 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KW551_RS19880 (4024739) 4024739..4025782 - 1044 WP_040153598.1 DUF2157 domain-containing protein -
KW551_RS19885 (4026452) 4026452..4027318 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
KW551_RS19890 (4027427) 4027427..4028854 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T209520 WP_004142563.1 NZ_CP079617:c4024554-4024237 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T209520 NZ_CP104123:1196780-1197091 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT209520 WP_004142561.1 NZ_CP079617:c4024244-4023957 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT209520 NZ_CP104123:1197088-1197507 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References