Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3212629..3213356 Replicon chromosome
Accession NC_004741
Organism Shigella flexneri 2a str. 2457T

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag S_RS17355 Protein ID WP_000550189.1
Coordinates 3213042..3213356 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag S_RS17350 Protein ID WP_000560266.1
Coordinates 3212629..3213045 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
S_RS17340 3207989..3210340 + 2352 WP_000695507.1 alpha-glucosidase -
S_RS17345 3210566..3212584 + 2019 WP_000121487.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
S_RS17350 3212629..3213045 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
S_RS17355 3213042..3213356 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
S_RS17365 3213641..3214777 - 1137 WP_000018656.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
S_RS17370 3214862..3215365 + 504 WP_005050988.1 M48 family metallopeptidase -
S_RS17375 3215442..3216134 + 693 WP_000942538.1 vancomycin high temperature exclusion protein -
S_RS17380 3216213..3217211 + 999 WP_005050984.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T20950 WP_000550189.1 NC_004741:c3213356-3213042 [Shigella flexneri 2a str. 2457T]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T20950 NC_004741:c3213356-3213042 [Shigella flexneri 2a str. 2457T]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT20950 WP_000560266.1 NC_004741:c3213045-3212629 [Shigella flexneri 2a str. 2457T]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT20950 NC_004741:c3213045-3212629 [Shigella flexneri 2a str. 2457T]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References