Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1625366..1625963 Replicon chromosome
Accession NZ_CP079606
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC15_AI1547PC

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KW550_RS08025 Protein ID WP_004142563.1
Coordinates 1625646..1625963 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KW550_RS08020 Protein ID WP_004142561.1
Coordinates 1625366..1625653 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW550_RS07990 (1621461) 1621461..1621709 + 249 WP_002893037.1 DUF1158 domain-containing protein -
KW550_RS07995 (1621727) 1621727..1622068 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KW550_RS08000 (1622099) 1622099..1623214 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
KW550_RS08005 (1623394) 1623394..1623978 + 585 WP_071579668.1 TetR/AcrR family transcriptional regulator -
KW550_RS08010 (1623975) 1623975..1624343 + 369 WP_040171587.1 MmcQ/YjbR family DNA-binding protein -
KW550_RS08015 (1624463) 1624463..1625101 + 639 WP_064277739.1 oxygen-insensitive NAD(P)H nitroreductase -
KW550_RS08020 (1625366) 1625366..1625653 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KW550_RS08025 (1625646) 1625646..1625963 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KW550_RS08030 (1626148) 1626148..1627191 - 1044 WP_040153598.1 DUF2157 domain-containing protein -
KW550_RS08035 (1627861) 1627861..1628727 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
KW550_RS08040 (1628836) 1628836..1630263 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T209491 WP_004142563.1 NZ_CP079606:c1625963-1625646 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T209491 NZ_CP104117:368812-369339 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT209491 WP_004142561.1 NZ_CP079606:c1625653-1625366 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT209491 NZ_CP104117:368539-368805 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References