Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 5014487..5015084 Replicon chromosome
Accession NZ_CP079599
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC10_CMC309MC

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KWY78_RS24135 Protein ID WP_004142563.1
Coordinates 5014487..5014804 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KWY78_RS24140 Protein ID WP_004142561.1
Coordinates 5014797..5015084 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY78_RS24120 (5010190) 5010190..5011617 - 1428 WP_004176980.1 MFS transporter -
KWY78_RS24125 (5011726) 5011726..5012592 + 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
KWY78_RS24130 (5013259) 5013259..5014302 + 1044 WP_004178895.1 DUF2157 domain-containing protein -
KWY78_RS24135 (5014487) 5014487..5014804 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KWY78_RS24140 (5014797) 5014797..5015084 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KWY78_RS24145 (5015352) 5015352..5016002 - 651 Protein_4735 oxygen-insensitive NAD(P)H nitroreductase -
KWY78_RS24150 (5016122) 5016122..5016490 - 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
KWY78_RS24155 (5016490) 5016490..5017071 - 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
KWY78_RS24160 (5017251) 5017251..5018366 + 1116 WP_020316605.1 MBL fold metallo-hydrolase -
KWY78_RS24165 (5018397) 5018397..5018738 + 342 WP_166689814.1 RamA family antibiotic efflux transcriptional regulator -
KWY78_RS24170 (5018756) 5018756..5019004 - 249 WP_002893037.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T209488 WP_004142563.1 NZ_CP079599:5014487-5014804 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T209488 NZ_CP104114:c4480851-4480744 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT209488 WP_004142561.1 NZ_CP079599:5014797-5015084 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT209488 NZ_CP104114:4480899-4480965 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References