Detailed information of TA system TA020946

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2039294..2039519
• Replicon: chromosome
• Accession: NC_004741
• Organism: Shigella flexneri 2a str. 2457T

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: S_RS11340
• Protein ID: WP_000813254.1
• Coordinates: 2039294..2039449 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2039461..2039519 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
S_RS11285	2034606..2034956	-	351	WP_005048975.1	IS66 family insertion sequence element accessory protein TnpB	-
S_RS11290	2034953..2035627	-	675	WP_004967157.1	IS66 family insertion sequence hypothetical protein	-
S_RS11295	2035726..2035941	-	216	WP_000839572.1	class II holin family protein	-
S_RS11320	2036742..2037425	-	684	WP_005049343.1	antiterminator	-
S_RS11325	2037422..2037787	-	366	WP_000140020.1	RusA family crossover junction endodeoxyribonuclease	-
S_RS11330	2037788..2038846	-	1059	WP_001265248.1	DUF968 domain-containing protein	-
S_RS26080	2038848..2039126	-	279	WP_011069426.1	hypothetical protein	-
S_RS11340	2039294..2039449	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	2039461..2039519	+	59	-	-	Antitoxin
S_RS11350	2040101..2040517	-	417	WP_005069274.1	hypothetical protein	-
S_RS11355	2040544..2040684	+	141	Protein_2057	DUF4224 domain-containing protein	-
S_RS11360	2040684..2041709	+	1026	WP_000533619.1	tyrosine-type recombinase/integrase	-
S_RS11370	2041945..2042742	+	798	WP_001325918.1	DgsA anti-repressor MtfA	-
S_RS11380	2043080..2044342	+	1263	Protein_2060	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ipaH9.8	2021895..2073955	52060

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T20946 WP_000813254.1 NC_004741:c2039449-2039294 [Shigella flexneri 2a str. 2457T]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T20946 NC_004741:c2039449-2039294 [Shigella flexneri 2a str. 2457T]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTTATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT20946 NC_004741:2039461-2039519 [Shigella flexneri 2a str. 2457T]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin