Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 335610..336196 Replicon chromosome
Accession NZ_CP079186
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC14_AI2040MC

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag KWZ53_RS01570 Protein ID WP_002920800.1
Coordinates 335828..336196 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag KWZ53_RS01565 Protein ID WP_004174006.1
Coordinates 335610..335831 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWZ53_RS01545 (331767) 331767..332693 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KWZ53_RS01550 (332690) 332690..333967 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
KWZ53_RS01555 (333964) 333964..334731 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
KWZ53_RS01560 (334733) 334733..335446 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
KWZ53_RS01565 (335610) 335610..335831 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KWZ53_RS01570 (335828) 335828..336196 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KWZ53_RS01575 (336469) 336469..337785 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
KWZ53_RS01580 (337892) 337892..338779 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
KWZ53_RS01585 (338776) 338776..339621 + 846 WP_004174009.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
KWZ53_RS01590 (339623) 339623..340693 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 320470..341430 20960


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T209434 WP_002920800.1 NZ_CP079186:335828-336196 [Klebsiella pneumoniae subsp. pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T209434 NZ_CP104108:3097008-3097226 [Cronobacter sakazakii]
ATGACGACCAAACCATTAACCAAAACCGATTATTTAATGCGACTGAGACGTTGTCAGACAATTGACACGCTTGAGCGCGT
TATTGAAAAAAATAAATATGAATTATCCGACAACGAACTGGCGGTATTTTATTCAGCGGCGGATCATCGTCTGGCTGAAC
TGACGATGAATAAACTCTACGATAAAATTCCGACCGCCGTGTGGAAGTTCGTTCGTTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT209434 WP_004174006.1 NZ_CP079186:335610-335831 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT209434 NZ_CP104108:3096607-3096981 [Cronobacter sakazakii]
ATGGACGAATACTCACCAAAACGGCATGATATAGCGCAGCTTAAATTTCTTTGCGAAAGCCTGTATCATGATTGCCTTGC
CACGCTCGGTGAAAACCAGCATGGCTGGGTTAACGACCCCACTTCTGCCGTTAACCTGCAACTCAACGACTTGATTGAGC
ATATTGCCAGCTTCGGCCTGAATTATAAAATTAAGCATGAAGATGACGCAGCTCTCATTGAGCAATTAGATGAATATCTG
GATGATACCTTTATGCTGTTCAGCAATTACGGTATCAATGCGCAAGATTTACAAAAATGGCGCAAGTCCGGAAACAGGCT
TTTCCGGTGTTTTGTAAACGCCAGCAAAGAAAACCCTGTAAGCTTGTCTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References