Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3989162..3989759 Replicon chromosome
Accession NZ_CP079179
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC13_AI2040PC

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KWY86_RS19600 Protein ID WP_004142563.1
Coordinates 3989442..3989759 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KWY86_RS19595 Protein ID WP_004142561.1
Coordinates 3989162..3989449 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY86_RS19565 (3985257) 3985257..3985505 + 249 WP_002893037.1 DUF1158 domain-containing protein -
KWY86_RS19570 (3985523) 3985523..3985864 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KWY86_RS19575 (3985895) 3985895..3987010 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
KWY86_RS19580 (3987190) 3987190..3987774 + 585 WP_071579668.1 TetR/AcrR family transcriptional regulator -
KWY86_RS19585 (3987771) 3987771..3988139 + 369 WP_040171587.1 MmcQ/YjbR family DNA-binding protein -
KWY86_RS19590 (3988259) 3988259..3988897 + 639 WP_064277739.1 oxygen-insensitive NAD(P)H nitroreductase -
KWY86_RS19595 (3989162) 3989162..3989449 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KWY86_RS19600 (3989442) 3989442..3989759 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KWY86_RS19605 (3989944) 3989944..3990987 - 1044 WP_040153598.1 DUF2157 domain-containing protein -
KWY86_RS19610 (3991657) 3991657..3992523 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
KWY86_RS19615 (3992632) 3992632..3994059 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T209426 WP_004142563.1 NZ_CP079179:c3989759-3989442 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T209426 NZ_CP104107:4823211-4823579 [Pseudomonas mosselii]
ATGGAATCGGACAGGCAACCCAAGCCGCTTTACTGGGTCGCAAGCAGCAAAAAGGATCTGCTGTGCCTCCCCGAGGACAT
CCAGCAAACCGTCGGCTTTGCTCTGCATCTGGCTCAGACGGGAAGCAGGCACCTCCAGGCGAAAGTCCTCAAAGGATTTT
CAGGCGCTGGCGTGCTCGAGGTCGTGGAGGACCACGACGGCAACACCTATCGCGCGGTCTATACCGTACGTCTTGGCGCT
GCGGTGTATGTCTTGCATTGCTTCCAGAAGAAGTCCAAGGCGGGCATCAGGACGCCCGAAACAGACATCGCCTTGGTGCG
CAAGCGATTGAAGCTTGCACAGGCCCACTCAGAAGGAATCCAGACATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT209426 WP_004142561.1 NZ_CP079179:c3989449-3989162 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT209426 NZ_CP104107:4823576-4823896 [Pseudomonas mosselii]
ATGAGCGAAATCGAACAGGGCAGCGGCAATGTCTACGCAGACCTCAACTACCCAGATGCAGACGAGATGCTGGTCAAGGC
ACGCCTGGCGAGCAAGATCTGCAGCATCATCAAGGCCCGTCATCTGACCCAGATGCAAGCCGCGCAAATCCTTGGCCTGC
CTCAGCCAAAAGTATCGGAAATGACTCGCGGAAAATTCCGTGGCATCAGCGAAAGCAAGATGATCGACTGCCTGGCACGC
CTTGGCCGCGACGTGCAGATCGTGGTCAAGGCCGCACCGCGTTCACGTCCCCAAGGGCGGGTGGAGGTCATCTTCGCCTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References