Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4021596..4022193 Replicon chromosome
Accession NZ_CP079172
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC11_S471PC

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KWY79_RS19825 Protein ID WP_004142563.1
Coordinates 4021876..4022193 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KWY79_RS19820 Protein ID WP_004142561.1
Coordinates 4021596..4021883 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY79_RS19790 (4017691) 4017691..4017939 + 249 WP_002893037.1 DUF1158 domain-containing protein -
KWY79_RS19795 (4017957) 4017957..4018298 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KWY79_RS19800 (4018329) 4018329..4019444 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
KWY79_RS19805 (4019624) 4019624..4020208 + 585 WP_071579668.1 TetR/AcrR family transcriptional regulator -
KWY79_RS19810 (4020205) 4020205..4020573 + 369 WP_040171587.1 MmcQ/YjbR family DNA-binding protein -
KWY79_RS19815 (4020693) 4020693..4021331 + 639 WP_064277739.1 oxygen-insensitive NAD(P)H nitroreductase -
KWY79_RS19820 (4021596) 4021596..4021883 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KWY79_RS19825 (4021876) 4021876..4022193 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KWY79_RS19830 (4022378) 4022378..4023421 - 1044 WP_040153598.1 DUF2157 domain-containing protein -
KWY79_RS19835 (4024091) 4024091..4024957 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
KWY79_RS19840 (4025066) 4025066..4026493 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T209407 WP_004142563.1 NZ_CP079172:c4022193-4021876 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T209407 NZ_CP104078:c26911-26495 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT209407 WP_004142561.1 NZ_CP079172:c4021883-4021596 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT209407 NZ_CP104078:c27138-26908 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References