Detailed information of TA system TA209372

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3557455..3558052
• Replicon: chromosome
• Accession: NZ_CP079155
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC08_CMC432MC

Toxin (Protein)

• Gene name: higB
• Uniprot ID: R4YIC5
• Locus tag: KWY73_RS17070
• Protein ID: WP_004142563.1
• Coordinates: 3557455..3557772 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: KWY73_RS17075
• Protein ID: WP_004142561.1
• Coordinates: 3557765..3558052 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KWY73_RS17055 (3553158)	3553158..3554585	-	1428	WP_004176980.1	MFS transporter	-
KWY73_RS17060 (3554694)	3554694..3555560	+	867	WP_004178894.1	helix-turn-helix transcriptional regulator	-
KWY73_RS17065 (3556227)	3556227..3557270	+	1044	WP_004178895.1	DUF2157 domain-containing protein	-
KWY73_RS17070 (3557455)	3557455..3557772	+	318	WP_004142563.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KWY73_RS17075 (3557765)	3557765..3558052	+	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
KWY73_RS17080 (3558320)	3558320..3558970	-	651	Protein_3338	oxygen-insensitive NAD(P)H nitroreductase	-
KWY73_RS17085 (3559090)	3559090..3559458	-	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
KWY73_RS17090 (3559458)	3559458..3560039	-	582	WP_043906838.1	TetR/AcrR family transcriptional regulator	-
KWY73_RS17095 (3560219)	3560219..3561334	+	1116	WP_020316605.1	MBL fold metallo-hydrolase	-
KWY73_RS17100 (3561365)	3561365..3561706	+	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
KWY73_RS17105 (3561724)	3561724..3561972	-	249	WP_002893037.1	DUF1158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T209372 WP_004142563.1 NZ_CP079155:3557455-3557772 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T209372 NZ_CP104049:c3494893-3494480 [Salmonella enterica subsp. enterica serovar Kentucky]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGTCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAAAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT209372 WP_004142561.1 NZ_CP079155:3557765-3558052 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT209372 NZ_CP104049:c3495140-3494874 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGACGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0M5MXH8

Antitoxin

• AlphaFold DB: A0A0S3DIQ1