Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 416422..417040 Replicon chromosome
Accession NC_004741
Organism Shigella flexneri 2a str. 2457T

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag S_RS02170 Protein ID WP_001291435.1
Coordinates 416422..416640 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag S_RS02175 Protein ID WP_000344797.1
Coordinates 416666..417040 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
S_RS02145 412314..412625 - 312 WP_000409911.1 MGMT family protein -
S_RS02150 413004..413357 + 354 WP_000878140.1 DUF1428 domain-containing protein -
S_RS02155 413399..414949 - 1551 WP_005098218.1 cyclic-guanylate-specific phosphodiesterase PdeB -
S_RS02160 415113..415583 - 471 WP_000136192.1 YlaC family protein -
S_RS02165 415699..416250 - 552 Protein_392 maltose O-acetyltransferase -
S_RS02170 416422..416640 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
S_RS02175 416666..417040 - 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
S_RS02180 417586..420735 - 3150 WP_001132484.1 multidrug efflux RND transporter permease subunit -
S_RS02185 420758..421951 - 1194 WP_011069267.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 410956..412284 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T20936 WP_001291435.1 NC_004741:c416640-416422 [Shigella flexneri 2a str. 2457T]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T20936 NC_004741:c416640-416422 [Shigella flexneri 2a str. 2457T]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT20936 WP_000344797.1 NC_004741:c417040-416666 [Shigella flexneri 2a str. 2457T]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT20936 NC_004741:c417040-416666 [Shigella flexneri 2a str. 2457T]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGCTCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAACGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References