Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4065667..4066264 Replicon chromosome
Accession NZ_CP079150
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC07_CMC432PC

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KWY72_RS19850 Protein ID WP_004142563.1
Coordinates 4065947..4066264 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KWY72_RS19845 Protein ID WP_004142561.1
Coordinates 4065667..4065954 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY72_RS19815 (4061747) 4061747..4061995 + 249 WP_002893037.1 DUF1158 domain-containing protein -
KWY72_RS19820 (4062013) 4062013..4062354 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KWY72_RS19825 (4062385) 4062385..4063500 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
KWY72_RS19830 (4063680) 4063680..4064261 + 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
KWY72_RS19835 (4064261) 4064261..4064629 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
KWY72_RS19840 (4064749) 4064749..4065399 + 651 Protein_3912 oxygen-insensitive NAD(P)H nitroreductase -
KWY72_RS19845 (4065667) 4065667..4065954 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KWY72_RS19850 (4065947) 4065947..4066264 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KWY72_RS19855 (4066449) 4066449..4067492 - 1044 WP_004178895.1 DUF2157 domain-containing protein -
KWY72_RS19860 (4068159) 4068159..4069025 - 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
KWY72_RS19865 (4069134) 4069134..4070561 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T209347 WP_004142563.1 NZ_CP079150:c4066264-4065947 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T209347 NZ_CP104037:110130-110420 [Rhizobium leguminosarum bv. viciae]
ATGACCGACATCATATTTTCTCCCGCTGCGCAAACCGATATTGACAAGATTTGGGATTACACAGCGACGAGTTGGAATGT
CGATCAGGCAGAGCGTTATGTTCAGGACATAAGAGACGCCTGTCAGGATCTGGCGGAGGGAACACGGATGAGCCGCCCCT
CAGATATTCGGAAAGGGTACAGAAAGGTCTCCGTTGGCTCTCATTTTCTGTATTTCCAAAGCAATGATGCGGGCCAGATC
ATCATCGTGCGCATCCTCCATCAACGAATGGACGTAGCCAAGCACCTGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT209347 WP_004142561.1 NZ_CP079150:c4065954-4065667 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT209347 NZ_CP104037:109885-110133 [Rhizobium leguminosarum bv. viciae]
ATGGCCAGAAGTACGTCCATATCGCTCGGCGAGCATTTCACCAGCTTCATCGATACGCAAGTTCAGGCTGGCCGCTATGG
TTCAGCCACTGACGTTGTTCGGGCCGGCCTTCGCCTACTTGAAGAACACGAAGCCAAAGTGAAGGCATTACAGGATGCCT
TGATTGAAGGCGAGGAATCCGGCCCTGCCACGCCCTTTGATTTCGATGCGTTCAACGCTCGCAAACGGGCGGCGTTCAAG
GCGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References