Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 301962..302656 Replicon chromosome
Accession NC_004741
Organism Shigella flexneri 2a str. 2457T

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag S_RS01555 Protein ID WP_001263491.1
Coordinates 302258..302656 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag S_RS01550 Protein ID WP_000554759.1
Coordinates 301962..302255 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
S_RS01530 297601..298098 + 498 WP_000006251.1 REP-associated tyrosine transposase RayT -
S_RS01535 298315..300027 - 1713 Protein_279 flagellar biosynthesis protein FlhA -
S_RS01540 299999..300784 + 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
S_RS01545 300855..301910 + 1056 WP_001226172.1 DNA polymerase IV -
S_RS01550 301962..302255 + 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
S_RS01555 302258..302656 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
S_RS01560 302666..303118 + 453 WP_001059860.1 GNAT family N-acetyltransferase -
S_RS01565 303364..304407 + 1044 WP_005053205.1 RNA ligase RtcB family protein -
S_RS01570 304469..305083 + 615 Protein_286 peptide chain release factor H -
S_RS01575 305140..306597 - 1458 WP_001293030.1 cytosol nonspecific dipeptidase -
S_RS01580 306859..307317 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gtrII / gtrB / gtrA 295042..344395 49353
- inside Prophage - gmhA/lpcA 220393..304407 84014


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T20934 WP_001263491.1 NC_004741:302258-302656 [Shigella flexneri 2a str. 2457T]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T20934 NC_004741:302258-302656 [Shigella flexneri 2a str. 2457T]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT20934 WP_000554759.1 NC_004741:301962-302255 [Shigella flexneri 2a str. 2457T]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT20934 NC_004741:301962-302255 [Shigella flexneri 2a str. 2457T]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References