Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4065657..4066254 Replicon chromosome
Accession NZ_CP079145
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC06_CMC387MC

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KWY71_RS19850 Protein ID WP_004142563.1
Coordinates 4065937..4066254 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KWY71_RS19845 Protein ID WP_004142561.1
Coordinates 4065657..4065944 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY71_RS19815 (4061737) 4061737..4061985 + 249 WP_002893037.1 DUF1158 domain-containing protein -
KWY71_RS19820 (4062003) 4062003..4062344 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KWY71_RS19825 (4062375) 4062375..4063490 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
KWY71_RS19830 (4063670) 4063670..4064251 + 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
KWY71_RS19835 (4064251) 4064251..4064619 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
KWY71_RS19840 (4064739) 4064739..4065389 + 651 Protein_3912 oxygen-insensitive NAD(P)H nitroreductase -
KWY71_RS19845 (4065657) 4065657..4065944 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KWY71_RS19850 (4065937) 4065937..4066254 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KWY71_RS19855 (4066439) 4066439..4067482 - 1044 WP_004178895.1 DUF2157 domain-containing protein -
KWY71_RS19860 (4068149) 4068149..4069015 - 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
KWY71_RS19865 (4069124) 4069124..4070551 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T209326 WP_004142563.1 NZ_CP079145:c4066254-4065937 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T209326 NZ_CP104030:355152-355499 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT209326 WP_004142561.1 NZ_CP079145:c4065944-4065657 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT209326 NZ_CP104030:355499-355798 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References