Detailed information of TA system TA209326

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 4065657..4066254
• Replicon: chromosome
• Accession: NZ_CP079145
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC06_CMC387MC

Toxin (Protein)

• Gene name: higB
• Uniprot ID: R4YIC5
• Locus tag: KWY71_RS19850
• Protein ID: WP_004142563.1
• Coordinates: 4065937..4066254 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: KWY71_RS19845
• Protein ID: WP_004142561.1
• Coordinates: 4065657..4065944 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KWY71_RS19815 (4061737)	4061737..4061985	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
KWY71_RS19820 (4062003)	4062003..4062344	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
KWY71_RS19825 (4062375)	4062375..4063490	-	1116	WP_020316605.1	MBL fold metallo-hydrolase	-
KWY71_RS19830 (4063670)	4063670..4064251	+	582	WP_043906838.1	TetR/AcrR family transcriptional regulator	-
KWY71_RS19835 (4064251)	4064251..4064619	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
KWY71_RS19840 (4064739)	4064739..4065389	+	651	Protein_3912	oxygen-insensitive NAD(P)H nitroreductase	-
KWY71_RS19845 (4065657)	4065657..4065944	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
KWY71_RS19850 (4065937)	4065937..4066254	-	318	WP_004142563.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KWY71_RS19855 (4066439)	4066439..4067482	-	1044	WP_004178895.1	DUF2157 domain-containing protein	-
KWY71_RS19860 (4068149)	4068149..4069015	-	867	WP_004178894.1	helix-turn-helix transcriptional regulator	-
KWY71_RS19865 (4069124)	4069124..4070551	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T209326 WP_004142563.1 NZ_CP079145:c4066254-4065937 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T209326 NZ_CP104030:355152-355499 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT209326 WP_004142561.1 NZ_CP079145:c4065944-4065657 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT209326 NZ_CP104030:355499-355798 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A0M5MXH8

Antitoxin

• AlphaFold DB: A0A0S3DIQ1