Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 3574919..3575505 Replicon chromosome
Accession NZ_CP079138
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC05_CMC387PC

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag KWY70_RS17070 Protein ID WP_002920800.1
Coordinates 3575137..3575505 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag KWY70_RS17065 Protein ID WP_004174006.1
Coordinates 3574919..3575140 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY70_RS17045 (3571076) 3571076..3572002 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KWY70_RS17050 (3571999) 3571999..3573276 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
KWY70_RS17055 (3573273) 3573273..3574040 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
KWY70_RS17060 (3574042) 3574042..3574755 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
KWY70_RS17065 (3574919) 3574919..3575140 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KWY70_RS17070 (3575137) 3575137..3575505 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KWY70_RS17075 (3575778) 3575778..3577094 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
KWY70_RS17080 (3577201) 3577201..3578088 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
KWY70_RS17085 (3578085) 3578085..3578930 + 846 WP_004181473.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
KWY70_RS17090 (3578932) 3578932..3580002 + 1071 WP_043906884.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3571999..3580739 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T209301 WP_002920800.1 NZ_CP079138:3575137-3575505 [Klebsiella pneumoniae subsp. pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T209301 NZ_CP104020:18831-19007 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT209301 WP_004174006.1 NZ_CP079138:3574919-3575140 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT209301 NZ_CP104020:c19169-18995 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References