Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1839250..1839847 Replicon chromosome
Accession NZ_CP079138
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC05_CMC387PC

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KWY70_RS08985 Protein ID WP_004142563.1
Coordinates 1839530..1839847 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KWY70_RS08980 Protein ID WP_004142561.1
Coordinates 1839250..1839537 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY70_RS08950 (1835330) 1835330..1835578 + 249 WP_002893037.1 DUF1158 domain-containing protein -
KWY70_RS08955 (1835596) 1835596..1835937 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KWY70_RS08960 (1835968) 1835968..1837083 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
KWY70_RS08965 (1837263) 1837263..1837844 + 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
KWY70_RS08970 (1837844) 1837844..1838212 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
KWY70_RS08975 (1838332) 1838332..1838982 + 651 Protein_1777 oxygen-insensitive NAD(P)H nitroreductase -
KWY70_RS08980 (1839250) 1839250..1839537 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KWY70_RS08985 (1839530) 1839530..1839847 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KWY70_RS08990 (1840032) 1840032..1841075 - 1044 WP_004178895.1 DUF2157 domain-containing protein -
KWY70_RS08995 (1841742) 1841742..1842608 - 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
KWY70_RS09000 (1842717) 1842717..1844144 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T209295 WP_004142563.1 NZ_CP079138:c1839847-1839530 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T209295 NZ_CP104014:c1086564-1086346 [Enterobacter sp. CP102]
ATGTCTGATAAACCATTAACCAAAATCGATTATTTGCTGCGTTTGCGACGTTGTCAGTCAATAGACACGCTTGAACGTGT
TATTGAAAAAAATAAATACGAGCTTTCTGATAATGAACTGGCTGTATTTTATTCAGCGGCTGACCACCGTCTTGCAGAAC
TCACCATGAATAAGTTGTATGACAAAATCCCTTCCTCTGTATGGAAGTTTGTCCGTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT209295 WP_004142561.1 NZ_CP079138:c1839537-1839250 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT209295 NZ_CP104014:c1086967-1086593 [Enterobacter sp. CP102]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCACGGCTGGGTGAATGATCCAACCTCTGCCATCAATTTACAGCTAAATGAGTTGATAGAAC
ATATCGCGACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAACTGATTGAGCAAATTGACGAATACCTG
GATGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAAAAATGGCGTAAATCGGGAAACCGCCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCGAACCCTGTTAGTTTATCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References