Detailed information of TA system TA209292

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 35808..36077
• Replicon: plasmid pAI1235M_P4
• Accession: NZ_CP079135
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC04_AI1235MC

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KWY69_RS28415
• Protein ID: WP_001372321.1
• Coordinates: 35952..36077 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	35808..35873 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KWY69_RS28390	31518..32045	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
KWY69_RS28395	32103..32336	+	234	WP_000006003.1	DUF905 family protein	-
KWY69_RS28400	32397..34420	+	2024	Protein_41	ParB/RepB/Spo0J family partition protein	-
KWY69_RS28405	34489..34923	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
KWY69_RS28410	34920..35639	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	35651..35875	+	225	NuclAT_0	-	-
-	35651..35875	+	225	NuclAT_0	-	-
-	35651..35875	+	225	NuclAT_0	-	-
-	35651..35875	+	225	NuclAT_0	-	-
-	35808..35873	-	66	-	-	Antitoxin
KWY69_RS29030	35861..36010	+	150	Protein_44	plasmid maintenance protein Mok	-
KWY69_RS28415	35952..36077	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KWY69_RS28420	36396..36692	-	297	Protein_46	hypothetical protein	-
KWY69_RS28425	36992..37288	+	297	WP_001272251.1	hypothetical protein	-
KWY69_RS28430	37399..38220	+	822	WP_001234445.1	DUF932 domain-containing protein	-
KWY69_RS28435	38517..39107	-	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
KWY69_RS28440	39442..39825	+	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KWY69_RS28445	40019..40690	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
KWY69_RS28450	40827..41054	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T209292 WP_001372321.1 NZ_CP079135:35952-36077 [Klebsiella pneumoniae subsp. pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T209292 NZ_CP104011:3478085-3478849 [Pseudomonas sp. GCEP-101]
ATGTCCTTCGCCGCCGGTGCCCGGCTCTGGGCGAACTACGAGGATTCGGTCGTCGCCCCCATGCGCGGGCGTCTGGTGCG
CCTGGTGGAAAGCCAGGGCCAGGTGGCCACGCTGCAACTGGTGGACACCCTGGAGGAACAGGCCCTGCTCGAGGAGTTGC
TGGAAACCAGCAAGCCACGCATGCCGCCGCCCGCCGAGCCGCTGCATTACCTGCTGAAGACGCCGTTCCGCTATCCGCCG
CTGCGCTGGGGTTCGCGCTTCGGCCGCCGGCATGAGCCGAGCCTGTTCTACGGTGCGCTGAAGCTGGAAACCGCCATGGC
CGAGTCGGCGTTCTACCGCTTCGTGCTGTGGGAGGGCATGGCGACGCCGCCGCCCAGCGGGCGAATCCTGTCCGAGCACG
CATCTTTCGAGGCGCGCTTCCAGGTGCAGAAGGGTGTGCGCCTGCACTTGCCGCCCTTCCGTGAACACGCGGCCCTGACC
GATCCGCAGGATTACAGCGTGACCCAGGGGCTGGGCAGCGCGATGCGCGAAGCGGGCATCGAAGCGTTCGAGTATCGCTC
GGCACGCTGTCCCCAGGGCGGGATCAACGTGGCGCTGTTCGTGCCCTCGGCCTTCACCGAGAAGCGCCCGCGCAACCTCA
TGCCCTGGCTGTGCGAAACCACTGCGGAGTACGTCGCCTTCAAGCATGCCCAGGTGCCCGACAGCCCGCGACTGTACCGG
CGCGAACAGTACTTGGTGAATGGCAGGCTGCCGCATCCGGCCTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT209292 NZ_CP079135:c35873-35808 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin